摘要
刺槐花叶病是我国北方刺槐（Ｒｏｂｉｎｉａｐｓｅｕｄｏａｃａｃｉａ）常见病害。１９８７～１９９５年对河南、河北、山东和北京部分地区刺槐进行调查，刺槐花叶病发病率为４％～８７．５％。该病害主要特征是：叶片出现浅绿与绿色相间带有疱疹的花叶症状，叶缘波状扭...
A virus isolated from a diseased black locust tree (Robinia pseudo accacia) in the suburb of Beijing was identified as peanut stunt virus (PSV),designated PSV Robinia isolate (PSV-R). PSV-R infected 14 of 24 species of plants in four families. The thermal inactivation point was 55-60℃. The dilution end point was 10 -4 -10 -5 and the longevity in vitro was 10 days. Aphid (Aphis craccivora Koch) transmitted the virus in a nonpersistent mamner. Virus particles were spherical with 30nm in diameter. An antiserum against PSV-R was produced with titers of 1∶1024 and 1∶256 in microprecipition and gel diffusion tests, respectively. Serologically PSV-R was closely related to PSV-Mi, distantly related to PSV-E. The molecular weight of PSV-R coat protein is about 26000D. PSV-R has four main components of RNAs with a small RNAs.
出处
《中国病毒学》
CSCD
1998年第3期271-274,共4页
Virologica Sinica
基金
国家自然科学基金
"九五"农业部重点科研项目
关键词
刺槐
花叶病毒病
花生矮化病毒
鉴定
Black locust, Mosaic disease, Peanut stunt virus, Identification
