摘要
马立克氏病病毒（Ｍａｒｅｋ’ｓｄｉｓｅａｓｅｖｉｒｕｓ，ＭＤＶ）是一种能诱导鸡淋巴组织增生或淋巴肿瘤的细胞结合性疱疹病毒，由此而引起的马立克氏病（Ｍａｒｅｋ’ｓｄｉｓｅａｓｅ，ＭＤ）也是迄今为止唯一可以利用疫苗进行有效控制的肿瘤性疾病。鉴于此，作为研...
Differentiation of strong virulent and vaccinal Marek's disease virus (MDV) was carried out by using DNA molecular hybridization. DNA probe was made of BamHI L fragment from MDV gene library (strain GA) by labelled with digoxigenin using random priming. And the complete nucleotide sequence of BamHI L fragment was further determined from subcloned DNAs by using double stranded sequencing method. The results of computer analysis showed that, (1) the entire DNA fragment is 2893 bp long and has a base composition of 55.91% G+C;(2) it has no any open reading frames (ORFs); (3) this DNA fragment is possibly involved in transcription of meq gene of MDV.
出处
《中国病毒学》
CSCD
1998年第3期268-270,共3页
Virologica Sinica
基金
农业部生物技术计划
