摘要
对单细胞微凝胶电泳（ＳＣＧＥ）技术的操作过程，技术原理以及实验操作过程中应注意的事项，进行了详细介绍和讨论；并应用ＳＣＧＥ技术研究了γ射线照射对人血淋巴细胞ＤＮＡ的损伤效应．结果表明，γ射线照射能引起细胞ＤＮＡ迁移长度增加，且呈显著地剂量效应关系．
The single cell microgel electrophoresis(SCGE)technique,its principle and noticeable details in the technique were described and disscussed.Effects of DNA damage in human blood lymphocytes induced by γ rays radiation were studied using this technique.It was shown that γ rays caused the increase of DNA migration length in the lymphocytes in the dose dependent manner.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第5期604-609,共6页
Chinese Journal of Biochemistry and Molecular Biology
