摘要
本研究选用枸杞体细胞胚发生体系中的继代愈伤组织（对照）、胚性愈伤组织和早期胚体为实验材料，提取细胞总ＲＮＡ，在１２种锚定真核生物ｍＲＮＡ３′末端的ＯｌｉｇｏｄＴ１２ＶＮ中，随机选用ＯｌｉｇｏｄＴ１２ＧＡ为引物合成了以上三种材料的ｃＤＮＡ第一链，以此ｃＤＮＡ为模板，用随机引物进行ＰＣＲ扩增，选择差别表达的片段。我们选用了ＯＰＡ、ＯＰＨ、ＯＰＫ和ＯＰＢ四组的６０个随机引物对所得的ｃＤＮＡ进行了ＰＣＲ扩增，得到了三个在体细胞胚发生早期组织中基因特异表达的片段。结果表明，在体细胞胚发生早期有胚胎发生特异性基因的表达，而且这种特异表达的基因在继代愈伤组织中没有表达。
Embryogenic calli and early embryo can be obtained from both auxin and auxin-free medium The analysis of differential gene expression in early somatic embryogenesis has been hindered by above-mentioned material The modifications of the recently described mRNA differential display method were reported and differential gene expression in early somatic embryogenesis was analyzed We have obtained three differential bands of cDNA in early somatic embryogenesis The results indicate that gene expression has temperal and spalil order in early somatic embryogenesis of Lycium barbarum L Plant somatic embryogenesis is the results of differential gene expression in cell
出处
《遗传》
CAS
CSCD
北大核心
1998年第5期16-19,共4页
Hereditas（Beijing)
基金
国家自然科学基金
甘肃省自然科学基金
关键词
MRNA
基因差别表达
PCR
体细胞胚发生
枸杞
mRNA,differential gene expression,PCR,somatic embryogenesis,Lycium barbarum L
