摘要
目的研究逍遥散防治肝郁脾虚型黄褐斑的作用及其机理。方法研究逍遥散预防和治疗肝郁脾虚型黄褐斑小鼠皮肤模型HMB45标记的皮肤黑色素细胞的病理形态,统计逍遥散防治肝郁脾虚型黄褐斑的疗效,并通过免疫组化法测定皮肤黑色素细胞NOS的表达和原位杂交法测定皮肤酪氨酸酶mRNA表达,探讨该药的作用机理。结果模型组HMB45标记的小鼠皮肤黑色素细胞内黑色素颗粒的阳性面积比例、IOD、MOD均较正常组显著升高(P<0.01),逍遥散治疗和预防组有明显下降趋势,表明逍遥散对于肝郁脾虚型黄褐斑小鼠具有良好的治疗和预防作用,其中治疗作用优于预防作用。预防组和治疗组中,NOS的表达与酪氨酸酶mRNA表达阳性细胞较模型组均有一定的减少。结论逍遥散对于肝郁脾虚型黄褐斑小鼠模型具有较好的预防和治疗作用,其作用机理可能是通过预防皮肤黑色素细胞的NOS和酪氨酸酶mRNA表达,以减少皮肤黑色素的生成。
Objective To study the function and its mechanism of Xiao Yao San in treatment of chloasma due to liver stagnation and spleen deficiency. Methods Study the pathological morphology of HMB45 labeled melanoeyte in the prevention and treatment of chloasma due to liver stagnation and spleen de- ficiency in mice by Xiao Yao San, make a statistic analysis on the efficacy of Xiao Yao San on ehloasma due to liver stagnation and spleen deficiency, determine the expression of melanocyte NOS by IHC method and the expression of tyrosinase mRNA by in situ hybridization and probe into the mechanism of this drug in treatment. Results The positive area proportion of melanin granules in HBM45 labeled melanoeytes, IOD and MOD in model group increased significantly compared with lhose in normal group （P 〈 0.01 ）. In treatment and prevention group with Xiao Yao San, eyelet index was in the tendency of decrease, which explained that Xiao Yao San played a good role in the treatment and prevention of chloasma due to liver stagnation and spleen deficiency in mice. The action of its treatment was superior to that of prevention. In prevention group and treatment group,melanocyte NOS and tyrosinase mRNA expressions decreased to a certain extent com- pared with model group. Conclusion Xiao Yao San play a good role in the prevention and treatment of chloasma due to liver stagnation and spleen deficiency in mice, which results probably from the prevention of NOS and tyrosinase mRNA expressions and the decrease of melanin generation.
出处
《世界中西医结合杂志》
2009年第12期867-869,共3页
World Journal of Integrated Traditional and Western Medicine
关键词
逍遥散
肝郁脾虚
黄褐斑
机理
Xiao Yao San
Liver stagnation and spleen deficiency
Chloasma
Mechanism
