摘要
选用４８只ＳＤ雄性大鼠随机等分３组，即Ａ组、Ｂ组和Ｃ组。再从每组中分出８只用于高温暴露，另８只用于室温对照，在ＡＩＮ－９３Ｍ饲料配方基础上，以碳酸锌为锌源，配制成Ａ、Ｂ、Ｃ组锌饲料。每千克饲料锌含量测定值：Ａ组为９２．２ｍｇ，Ｂ组４５．６１ｍｇ，Ｃ组２１．７０ｍｇ。根据动物分组，饲以各组大鼠相应水平锌饲料１４天后，将用于高温暴露的大鼠置于高温室（Ｔｄｂ４０℃，Ｔｗｂ３０．８℃）连续暴露３小时。用原子吸收分光光度计火焰法测定大鼠海马、大脑皮层和小脑Ｚｎ、Ｃｕ、Ｆｅ含量。实验结果发现：①高温暴露可使海马铜含量显著增加：②高温暴露后，海马铜含量比小脑和大脑皮层铜含量高，而在室温对照大鼠，小脑铜含量却比大脑皮层和海马铜含量高。③摄入不同水平饲料锌主要影响小脑铜的含量，对铁的影响较小。④中。
Forty-eight SD male rats were randomly divided into group A, B and C. Rats in each group were also divided into 2 parts. One part was used for heat exposure and the other was used for room temperature control. On the basis of AIN93m diet composition, group A, B and C zinc diets were prepared by adding or reducing zinc carbonate. Zinc contents of group A, B and C zinc diet per kilogram were 922 mg, 4561 mg and 2170 mg respectively. 14 days later the rats were exposed to high temperature (Tdb 40℃, Twb 308℃) for 3 hours. The Contents of Zn, Cu and Fe in hippocampus, cerebral cortex and cerebellum of rats were determined by atomic absorption spectrophotometer. The results revealed that ① hyperthermal exposure could increase Cu content in rat hippocampus significantly, ②after heat exposure, Cu content in hippocampus was higher than in cerebral cortex and cerebellum. Cu contents in cerebellum of room temperature control rat were higher than cerebral cortex and hippocampus, ③ intakes of different zinc level diets chiefly influenced content of Cu rat cerebellum and slightly affected iron content in it, ④high, middle level zinc diet intake could reduce Cu content in heatstressed rat cerebellum.
出处
《卫生研究》
CAS
CSCD
北大核心
1998年第5期320-322,共3页
Journal of Hygiene Research
基金
国家教委博士研究生基金
关键词
膳食
锌
热应激
脑区
微量元素
\ diet zinc, heat exposure, brain domain, trace element
