摘要
研究羟基喜树碱体外诱人胰腺癌细胞株SW－1990凋亡的作用。方法：以不同浓度的羟基喜树碱处理在体外培养的胰腺细胞，以电子显微镜、流式细胞仪和原位末端标记分析和检测细胞凋亡。结果：在0．05mg/ml浓度下，羟基喜树碱作用于体外培养的胰腺癌细胞20小时后，肿瘤细胞的凋亡率在13．2％－15．3％之间，主要以早、中期的表现为主：在0．lmg／ml浓度下，作用20小时后，凋亡率在26．l％－30．4％之间，凋亡形态学表现主要以晚期为主。结论：羟基喜树碱可作为细胞凋亡诱导剂用于胰腺癌治疗。
Objective to study the effect of hydroxyl camptothecinum on human pancreatic cancer cell apoptosis in vitro．Methods: SW-1990 cell line were treated with hydroxyl camptothecinum at different concentrations. cell apoptosis was evaluated by electric microscopy, flow cytometry and in situ TUNEL (TdT-mediated dUTP-X nick end labeling). Results The apoptosis rate of Pancreatic cell treatet with hydroxyl camptothecintm at the concentration of 0.05mg/ml for 20 hours is between 13.2% ~ 15. 3 %, mainly at the early and middle morphological stage. At the concentration Of 0.lmg/ml, the rate is between 26.1% ~ 30.4%, mainly at late stage. Conclusion hydroxyl camptothecinum is a potential agent for pancreatic cancer chemotherpy.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1998年第5期329-331,共3页
Cancer Research on Prevention and Treatment
