摘要
目的观察以"浊毒"立论的肝复健方对猪血清诱导的大鼠肝纤维化(HF)肝脏转化生长因子-β1(TGF-β1)及其Ⅱ型受体(TβRⅡ)mRNA水平和Smad2/3、Smad4、Smad7蛋白表达的影响。方法SD大鼠腹腔注射猪血清0.5 mL,每周2次,连续8周制备HF模型,每天灌胃分别给予不同剂量的肝复健方(15.0、22.5、30、0 g/kg)和秋水仙碱(0.154 g/kg)治疗至12周末。实验结束后,RT-PCR法检测肝组织TGF-β1及其受体TβRⅡmRNA的表达,Weston-blot法检测肝组织Smad2/3、Smad4、Smad7蛋白的表达。结果与正常对照组相比,模型组TGF-β1、TβRⅡ及Smad2/3、Smad4表达明显增强,Smad7表达明显下降;经肝复健方治疗后大鼠肝脏TGF-β1及其受体TβRⅡ表达均比模型组减弱,Smad2/3、Smad4的表达减弱而Smad7蛋白的表达增强。结论肝复健方能抑制TGF-β1及其受体TβRⅡmRNA的表达,下调Smad2/3、Smad4蛋白表达,上调Smad7蛋白的表达。肝复健方对大鼠HF肝脏TGF-β/Smad信号通路有明显的影响,这可能是其治疗HF的机制之一。
Objective To observe the influences of Ganfujian Formula on the expressions of transforming growth factor-β1（TGF-β1）,TβRII-mRNA,Smad2/3,Smad4 and Smad7 in rats with hepatic fibrosis induced by porcine serum.Methods The hepatic fibrosis model was established in SD rats by the intraperitoneal injection of porcine serum（0.5 mL,twice a week for 8 weeks）.The rats then were given Ganfujian Formula orally in different doses（1.50 g/kg,2.25 g/kg and 3.0 g/kg） and colchicines （0.154 g/kg） respectively until the end of 12th week.The expressions of TGF-β1 and TβRII-mRNA were detected by using RT-PCR and the expressions of Smad2/3,Smad4 and Smad7 were detected by applying Western blotting method.Results In the model group,compared with the normal control group,the expressions of TGF-β1 and TβRII-mRNA,Smad2/3 and Smad4 increased significantly and Smad7 expression decreased significantly.After the treatment with Ganfujian Formula the expressions of TGF-β1 and TβRII-mRNA were reduced in the treatment group compared with the model group. Ganfujian Formula regulated downward the expressions of Smad2/3 and Smad4,and regulated upward Smad7 expression at the same time.Conclusion Ganfujian Formula can inhibit the expressions of TGF-β1 and TβRII-mRNA,regulates downward the expressions of Smad2/3 and Smad4,and regulates upward Smad7 expression.It may be its one of the mechanisms for curing hepatic fibrosis that it has an obvious influence on hepatic TGF-βand Smad signal pathway.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第11期755-758,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
河北省科技厅资助项目(No.09276102D-11)
