摘要
目的：建立龙虾中草酸含量测定的离子色谱检测方法。方法：选用Shodex IC SI-52E阴离子分析柱,3.6 mmol的NaCO3为流动相,流速为0.7 ml/min,电导检测器。样品浸泡液经超滤后直接进样检测。结果：草酸浓度在0.05～0.8μg/ml范围内呈线性相关,回归方程为：Y=-202289X-4105.5,相关系数r=0.9995。该法的最低检出浓度为0.01μg/ml,回收率为95.6%～97.7%。通过检测,所有抽检样品均含有一定量的草酸,其分布范围在0.32～1.46 mg/kg之间,平均值为0.85 mg/kg。结论：该法操作简便,结果准确,分离度好,可用于龙虾中草酸含量的检测。
Objective：An ICS method was applied to determine oxalic acid in crawfish.Methods：The method was based on ICS separation under the condition of isocartic eluent with 3.6 mmol NaCO3 and flow rate of 0.7 ml/min by using of the Shodex IC SI-52E analytic column and suppressed conductivity detector.Results：This method has a good relativity（r=0.9995）,and the aclibration curve showed good linearity in range of 0.05～0.8 μg/ml.The mean recovery was between 95.6% and 97.7%,and the detection limit was 0.01 μg/ml.The trace oxalic acid was founded in every sample,and the range is between 0.32 mg/kg and 1.46 mg/kg,the average is 0.85 mg/kg.Conclusion：The advantages of this method are simple operation and high sensitivity,it can be applied and suitable for trace analysis of oxalic acid in crawfish.
出处
《中国卫生检验杂志》
CAS
2009年第12期2835-2836,2888,共3页
Chinese Journal of Health Laboratory Technology
关键词
龙虾
草酸
离子色谱
Crawfish
Oxalic acid
Ion chramatography
