摘要
运用基于GC/MS的代谢组学方法,以受精卵在双酚A(BPA)中暴露4d后的斑马鱼胚胎为对象,研究了其体内挥发性和半挥发性代谢组分变化.暴露浓度设定为0,0.5,1.5,4.5mg/L,包括USEPA假定的"安全水平"(1.5mg/L).结果表明,1.5mg/LBPA暴露能使斑马鱼体内花生四烯酸和胆固醇的相对百分含量极显著降低,饱和脂肪酸、氨基酸、葡萄糖和肌糖的相对百分含量显著升高.因此,1.5mg/LBPA能干扰斑马鱼胚胎期的正常代谢.
Metabonomics approach based on the GC-MS technique was used to characterize the perturbation of volatile and semi-volatile components in zebrafish embryos induced by bisphenol A （BPA） for 4 d since fertilization. The exposure concentrations of BPA were 0, 0.5, 1.5 and 4.5mg/L, including assumed＂safe level＂（1.5mg/L） that stated by US EPA. The relative proportions of arachidonic acid and cholesterol were decreased dramatically, and those of saturated fatty acid （SAF）, amino acid, glucose and inositol were increased significantly. Therefore, 1.5 mg/L BPA would disturb the regular metabolizing of zebrafish embryos.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2009年第12期1318-1322,共5页
China Environmental Science
基金
国家自然科学基金资助项目(30470319)
