摘要
目的研究浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达的意义。方法利用免疫组织化学链霉亲和素生物素法(labelled streptavidin-biotin method,LSAB)方法检测61例浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)、71例浸润性导管癌(invasive ductal carcinoma,IDC)和27例髓样癌(medullary carcinoma,MC)3种乳腺癌中浸润T淋巴细胞亚群分布以及Th细胞因子的表达。T淋巴细胞组间比较采用Kruskal-WallisTest,组内比较采用Wilcoxon Signed RanksTest;组间Th细胞因子的比较采用χ2检验。结果CD3、CD8和CD4均表达于淋巴细胞胞膜。CD3+T细胞在3组间差异无统计学意义(P=0.735)。CD8+T和CD4+T细胞在3组间差异均有统计学意义(P=0.000,P=0.012)。Th细胞因子主要表达于肿瘤细胞和淋巴细胞的胞质。IL-2表达在IMPC组最低(25/61,41.0%),其次是IDC组(33/71,46.5%),最高是MC组(20/27,74.1%),3组间差异有统计学意义(P=0.014);IFN-γ的表达类似于IL-2,IMPC组最低(39/61,63.9%),其次是IDC组(57/71,80.3%),最高是MC组(24/27,88.9%),3组间差异有统计学意义(P=0.019);IL-4的表达在IDC组最高(42/71,59.2%),其次是IMPC组(19/61,31.1%),最低是MC组(5/27,18.5%),3组间差异有统计学意义(P=0.000);IL-10的阳性率在IMPC组是87.8%(47/61),在IDC组是87.3%(62/71),在MC组是88.9%(24/27),3组间差异无统计学意义(P>0.050)。结论浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达不同。
Objective To study the expression of tumor-infiltrating lymphocytes （TILs） and T helper cytokines in invasive micropapillary carcinoma （IMPC）, invasive ductal carcinoma （IDC） and medullary carcinoma （MC） of the breast. Methods Three T-cell markers including pan-T cell marker （CD3）, T-helper cell marker （CD4）, cytotoxie T cell marker （CD8）, and four T-helper cytokines including interleukin-2 （IL-2）, interferongamma （IFN-γ）, interleukin-4 （IL-4）, and interleukin-10 （IL-10） were tested immunohistochemically in the three groups, （IMPC n=61, IDC n=71, and MC n=27） on formalin-fixed, paraffin embedded tumors. Kruskal-Wallis Test and Wilcoxon Signed Ranks Test were used for analysis of TILs;chi-square test was used for analysis of T helper cytokines. Results Markers, CD3, CD4 and CDS, all expressed in the cell membrane of lymphocytes. There was no statistical difference in CD3＋T cells number between the three groups （P= 0. 705）; CD8^＋ T cells and CD4^＋ T cells were statistically different between the three groups （P=0. 000,P=0. 012）. T helper cytokines expressed in cytoplasm of tumour cells and lymphocytes. The expression of IL-2 was the highest in the MC group （20/27,74. 1%）, followed by the IDC group （33/71,46. 5%）, and the lowest in the IMPC group （25/61, 41.0%）, there was a statistical difference between the three groups（P =0. 014）. The expression of IFN-γ was the highest in the MC group （24/27,88. 9%） followed by the IDC group（57/71,80. 3%）, and the lowest in the IMPC group（39/61,63.9%）, there was a statistical difference between the three groups（P = 0. 019）. The positive expression of IL-4 was the highest in the IDC group （42/71,59. 2%）, followed by the IMPC group （19/61, 31.1%） and the lowest in the MC group （5/27, 18. 5%）, there was a statistical difference between the three groups （P=0. 000）. The positive rate of IL-10 expression was 87.8%（47/61）in the IMPC group, 87.3%（62/71） in the IDC group, and 88. 9%（24/27）in the MC group, with no statistical difference between the three groups. （P〉0. 050）. Conclusion The expressions of infiltrating T lymphocytes and T helper cytokines were different in different types of breast carcinomas, which may be responsible for different biologic behavior.
出处
《中华乳腺病杂志（电子版）》
CAS
2009年第6期28-33,共6页
Chinese Journal of Breast Disease(Electronic Edition)
基金
国家自然科学基金资助项目(30600225)
教育部长江学者奖励计划乳腺癌创新团队(IRT0743)
国家"863"计划项目(2006AA02A249)
国家"973"计划项目(2009CB521700)
关键词
乳腺肿瘤
浸润T淋巴细胞
TH细胞因子
Breast neoplasms； Infiltrating T lymphocytes
T helper cytokines
