摘要
目的探讨硝酸亚铈体外毒性、抑瘤率及其对细胞周期的影响。方法采用MTT法检测硝酸亚铈对正常细胞系FL、L929体外毒性,对肿瘤细胞系Hela、SGC-7901、B16、Lewis、K562、H22的体外抑瘤效应。采用流式细胞仪检测硝酸亚铈对SGC-7901的细胞周期影响。结果硝酸亚铈在0.64 mmol/L以下时对FL、L929无任何毒副作用;硝酸亚铈在0.64 mmol/L以上时对以上6种肿瘤细胞均有明显的抑制增殖作用,在0.08 mmol/L时对HeLa、SGC-7901、B16及K562肿瘤细胞表现出显著的抑制增殖作用,在0.010、.04 mmol/L时,对H22亦有显著抑制作用;0.08mmol/L硝酸亚铈作用48 h,能显著延长SGC-7901细胞株的G0/G1期,缩短S期;1.28 mmol/L硝酸亚铈作用48 h,除了能够显著延长SGC-7901细胞株的G0/G1期,显著缩短S期以外,还能延长G2/M期。结论硝酸亚铈对正常细胞具有低毒性和高度选择性,在体外通过影响细胞周期来抑制多种肿瘤细胞的生长和增殖。
Objective To investigate the cytotoxicity on normal cell lines of cerium nitrate, the inhibitory effect on tumor cell lines and the effect on cell cycle in vitro. Methods The cytotoxicity of cerium nitrate on FL, L929 and inhibition rate on Hela, SGC-7901, B16, Lewis, K562, and H22 in vitro were detected with the method of MTT. The effect of cerium nitrate on cell cycle of SGC-7901 was studied by one-fluorescence dying method on flow cytometer. Results Cerium nitrate did not show the toxic activities on FL and L929 cell lines when the dose of cerium nitrate was below 0.64 mmol/L. When the dose of cerium nitrate was above 0.64 mmol/L, it could noticeably inhibit the growth and proliferation of tumor cells （Hela, SGC-7901, B16, Lewis, K562, H22） in vitro. 0.08 mmol/L cerium nitrate inhibited the growth and proliferation of turner cells including HeLa, SGC-7901, B16 and K562 ; cerium nitrate had a noticeably inhibitory effect on the growth and proliferation of H22 cell lines in vitro on 0.04 mmol/L and 0.01 mmol/L. Cerium nitrate affected tumor cell lines for 48 h, cerium nitrate could delay G0/ G1 and shortened S of cell cycle at the dosage of 0.08 mmol/L; at the same time cerium nitrate could delay G0/G1, G2 ＋M and shorten S of cell cycle at the dosage of 1.28 mmol/L. Conclusion Cerium nitrate has the property of low toxicity and high selectivity on normal cells, but it can inhibit growth and proliferation of many kinds of tumor cell lines by adjusting cell cycle.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第6期709-711,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
硝酸亚铈
细胞周期
细胞培养
cerium nitrate
cell cycle
cellular culture
