摘要
目的观察紫杉醇(PA)对体外培养的Bxpc-3细胞凋亡的影响。方法体外培养Bxpc-3人胰腺癌细胞,采用MTT法检测不同浓度PA对Bxpc-3细胞的抑制情况,采用流式细胞术检测Bxpc-3细胞的周期及凋亡率。加入正常的培养液作为对照。结果与对照相比,不同浓度PA(100,50,25mg/ml)作用24h可使Bxpc-3细胞生长明显抑制,抑制率呈浓度依赖关系;Bxpc-3细胞在PA100,50mg/ml作用下,G0/G1期细胞比例增加,S期细胞比例和增殖指数(PI)下降;PA100,50mg/ml作用48h后凋亡指数(AI)增高,AI/PI比例分别是对照组的6倍和2.5倍。结论PA对Bxpc-3细胞生长有抑制作用,可诱导人胰腺癌细胞Bxpc-3细胞凋亡。
Objective To explore the effect of paclitaxel （PA） on apoptosis of cultured human pancreatic carcinoma ceils Bxpc-3 in vitro. Methods Bxpc-3 cells were cultured separately with RPMI1640 culture medium（ control group）and with 100,50,25 mg/ml of PA（ PA groups）. Bxpc-3 cell proliferation was detected with the modified MTr method. The Bxpc-3 cell DNA ploidy distribution and apoptotic rate were measured by flow cytometry. Results Compare with the controls,the Bxpc-3 cell proliferation was significantly inhibited in a dose dependent manner after treated with PA （100,50,25 mg/ml）for 24 h;the percentage of Bxpc-3 cell population in the G0/G1 phase increased after treated with 100,50 mg/ml PA, but reduced in S phase, and proliferation index （PI） also reduced. Cell apoptosis index（AI）was higher in 100,50 mg/ml PA 48 h group than in control group. AI/PI in 100,50 mg/ml PA 48 h group were 6 and 2.5 times as much as that in control group. Conclusion PA can inhibit cell proliferation and induce cell apoptosis of human pancreatic carcinoma cells Bxpc-3.
出处
《山西医科大学学报》
CAS
2009年第12期1074-1076,共3页
Journal of Shanxi Medical University
