摘要
利用AFLP技术,选择EcoRⅠ/MseⅠ这一酶切组合,应用9对(EcoRⅠ+3)/(MseⅠ+3)引物组合进行选择性扩增,检测16种箬竹和5个形态相似的外源竹种的基因组DNA多态性。在21个样品基因组中共获得1367条清晰的谱带,其中共有条带115条,特异性带273条,特异性缺失条带72条。在16个箬竹样品基因组中共获得1193条清晰的谱带,其中共有条带190条,特异性带177条,特异性缺失条带98条。这些特异性带或特异性缺失条带可作为识别不同竹种的分子标记。聚类结果表明:所有的箬竹属植物聚成一类,其他5种外源植物聚成一类。此外,AFLP分析提供的分子证据,支持耿氏分类系统(1996),将天目箬竹归入阔叶箬竹中。
Amplified fragment length polymorphism（AFLP）was used to study DNA diversity of 16 species in Indocalamus and other 5 similar bamboo species.Genomic DNA was digested with EcoRⅠ and MseⅠ enzymes and amplified with 9（EcoRⅠ＋3）/（MseⅠ＋3）primer combinations.In the 21 samples,9 EcoRⅠ-MseⅠ AFLP primer combinations produced 1 367 legible bands,of which there were 115 common bands,273 specific bands and 72 specific missing bands.In the 16 samples of Indocalamus,1 193 legible bands were obtained by using 9 primer combinations,with 190 common bands,177 specific bands and 98 specific missing bands.All the specific bands and specific missing bands would be used as molecular markers to identify these species.The AFLP markers indicated that I.migoi and I.latifolius were the same species.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2009年第11期32-35,共4页
Scientia Silvae Sinicae
基金
"十一五"科技支撑项目(2006BAD19B0202)
林业科学技术研究项目"重要竹种基因组测序及功能基因开发"(2007-01)
国际竹藤网络中心基本科研业务专项资金项目(06/07-C22)
