摘要
利用流式细胞光度术对草莓３个二倍体、１个五倍体、２个八倍体试管苗的细胞核ＤＮＡ的相对含量进行测定。结果表明，随着倍性水平的增加，细胞核ＤＮＡ相对含量随之成倍增加，说明可利用流式细胞光度术测定细胞核ＤＮＡ含量来进行倍性鉴定；利用这一技术对草莓几个杂交后代及诱变植株的倍性水平进行了鉴定，发现一诱变植株存在两群核ＤＮＡ相对含量相差近一倍的细胞，初步认为这一诱变植株为倍性嵌合体，该结果进一步证明这种方法的可靠性。
The relative nuclear DNA of diploid,pentaploid and octoploid was measured by flow cytometry.The results showed that cell nuclear DNA content increased with ploidy increasing.Therefore,the flow cytometerg could be used for ploid identification of some cross progenies, and differentiation of induced various plants,with this method found that there were two cell groups in which nuclear DNA content diverged almost a half, and this induced plant could be a ploid chimeral.The result of the method offered a better proof of reliability.
出处
《西北农业大学学报》
CSCD
北大核心
1998年第4期45-48,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
山东省自然科学基金
