摘要
目的:探讨病毒性肝炎患者细胞凋亡情况及其临床意义。方法:应用DNA缺口末端标记技术检测了28例各型病毒性肝炎肝组织细胞凋亡密度和分布,以8例正常肝组织为对照。结果:DNA缺口末端标记技术能较好地检测肝细胞凋亡,阳性细胞多单个散在于肝小叶内,以门管区周围较多见。比较各型肝炎的凋亡细胞密度发现,重型肝炎的凋亡细胞密度高于慢性肝炎和肝硬变(P均<0.01);慢性肝灸和肝硬变的凋亡细胞密度高于急性肝炎(P<0.01)。结论:细胞凋亡可能与病毒性肝炎的慢性化有关;也可能是重型肝炎的发病机制之一。
Aim: To investigate the apoptosis in liver tissues from patients with viral hepatitis B and its clinical significance. Methods: The density and distribution of the apoptotic cells were observed with DNA nick end labeling (DNEL) technique in situ in liver tissues from 28 patients with viral hepatitis B. 8 normal liver tissues were used as controls. Results: DNEL technique could detect the apoptotic cells in paraffin-embeded liver tissues satisfactorily. The positive cells were scattered in the introlobular areas, but they were more commonly seen in periportal areas. The density of the apoptotic cells in severe viral hepatitis was significantly higher than that in chronic hepatitis and liver cirrhosis (both P<0. 01). Their density in chronic hepatitis and cirrhosis were much higher than that in acute viral hepatitis (P<0. 01). Conclusion: The apoptosis may be related with chronicity of viral hepatitis and may play important role in the m chanism of severe viral hepatitis.
出处
《中西医结合肝病杂志》
CAS
1998年第3期136-138,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
病毒性肝炎
细胞凋亡
DNEL
检测
Viral Hepatitis Apoptosis DNA Nick End Labeling Techinique
