摘要
本文报道了应用酶联免疫吸附试验(ELISA)检测包虫病人血清中抗包虫特异性免疫球蛋白(IgG和IgM)的水平并对其临床意义进行了初步探讨。48例正常人血清特异性IgG和IgM的假阳性检出串均为0％;24例囊尾蚴病人血清特异性抗包虫IgG和IgM的假阳性检出效分别为:33.4％;4.16％;42例包虫病患者血清特异性IgG和IgM的阳性检出率分别为:80.95％,66.67％;两者之差经统计学处理具有显著意义(p<0.05)。肝包虫特异性IgG的阳性检出率较肺包虫高,肺包虫特异性IgM的阳性检出率较肝包虫高。这提示患者对包虫的免疫应答和其寄生的位置有关;诱导产生的特异性免疫球蛋白主要为IgG类,特异性IgM的水平与包虫囊壁的完整与否以及检测方法的灵敏性、特异性有关。
The specific anti-hydatid immunoglobulin (IgG and IgM) in sera from 42
patients confirmed surgically with hydatid cysts were detected by ELISA tests using purified sheep hydatid cyst fluid antigens. The technique was easy to perform and our results suggested that the test was sensitive and specific.The results showed that no false positivity of specific IgG and IgM were found in sera from 48 health donors. Cross reactions between the antigens and antibodies of hydatid disease and cysticercosis were show to be present mainly in the IgG and only to a much smaller extent in IgG.Specific IgG antibodies were mainly present in patients with heptic hydatidosis and secondarily pulmonary hydatidosis. The variation of the specific IgM level is obvious (1) during different stages of hydatidosis, (2) probably undly different physical conditions.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1990年第3期192-194,共3页
Immunological Journal
关键词
包虫病
免疫球蛋白
Hydatidosis, Hydatid cyst, Immunodiagnosis, ELISA, IgG, IgM
