摘要
本文报告一种检测人PBMC自发细胞毒作用的简便方法。以SRBC作靶细胞,与PBMC一起孵育后,SRBC破坏释放出Hb,Hb催化H_2O_2对邻苯二胺的氧化作用,从而通过显色反应即能反映PBMC的自发细胞毒活性。文中拥讨了SRBC的适宜浓度、效靶比、最佳作用时间及孵育温度等主要实验条件。
convenient metnoa to assay spontaneous cytotozicity ot numan peripnerai blood mononuclear cells (PBMC) was reported here. Using sheep erythrocyte (SRBC) as target, the SRBC, when cocultured with PBMC, was cytolyzed and the released Hb then catalyzed H2O2 to oxidize o-pheno-diamine. Thus the spontaneous cytotoxicity of PBMC to SRBC can be evaluated by colorimetry. The main experiment conditions, adequate amount of SRBC, ratio of effector to target, optimal coculturing time and temperature, were studied in this report.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1990年第2期130-131,125,共3页
Immunological Journal
