摘要
用ＨＰＬＣ法测定黄草乌中滇乌碱的含量。色谱条件：采用氰基硅烷键合硅胶为填充剂装柱；流动相：甲醇－水－氨水（３５：６５：０．５）；检测波长：２６０ｎｍ，在此检测条件下，滇乌碱与其相邻峰能达到基线分离；滇乌碱的检测线性范围２．６～１５．６μｇ，相关系数ｒ＝０．９９９９；平均回收率９７．６％（ｎ＝５）。
The assay of Yunaconitine in Aconitum vilmorinianum kom.with bonded cyano-silica column,mobile phase was MeOH-H_2O-NH_3·H_2O(35∶65∶0.5),detcting wavelength was 260 nm; the baseline resolution of Yunaconitine was obtained. Its linear range was 2.6～15.6μg; related coefficient was 0.9999;the average recovery was97.6%(n=5).
出处
《中药新药与临床药理》
CAS
CSCD
1998年第3期174-176,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
黄草乌
滇乌碱
高效液相色谱法
化学成分
@ Aconitum vilmorinianum kom./ chemistry
@ Yunaconitine/ analysis, HPLC
