摘要
目的：研究庆大霉素（ＧＭ）引起大鼠急性肾功能衰竭（ＡＲＦ）后，人参总皂甙（ＧＳ）能否减轻ＧＭ的进一步肾损害，促进肾小管的修复。方法：雄性Ｗｉｓｔａｒ大鼠３２只随机分成３组：正常对照组（Ⅰ组）腹腔注射生理盐水７ｄ，模型组（Ⅱ组）腹腔注射ＧＭ７ｄ，在随后７ｄ中自由饮水，ＧＳ治疗组（Ⅲ组）腹腔注射ＧＭ７ｄ后，给予ＧＳ灌胃７ｄ。结果：ｄ１５显示Ⅱ组中血肌酐水平、血清和肾皮质匀浆中丙二醛（ＭＤＡ）含量均比Ⅰ、Ⅲ组显著升高（Ｐ＜００１），同时血清和肾皮质匀浆中超氧化物歧化酶（ＳＯＤ）活性比Ⅰ、Ⅲ组显著下降（Ｐ＜００１）；但Ⅲ组血肌酐升高与Ⅰ组无显著性差异，血清和肾皮质匀浆中ＭＤＡ升高与ＳＯＤ下降较Ⅰ组有显著性差异（Ｐ＜００１）。病理检查Ⅲ组也比Ⅱ组损害轻、修复快。结论：反应性氧代谢产物（ＲＯＭ）的增多及肾脏抗氧化能力的下降参与了大鼠ＧＭ肾损害，ＧＳ能通过清除ＲＯＭ减轻肾损害，促进肾小管及肾功能的恢复。
Objective:To study whether ginsenoside(GS) could ameliorate the harmful effects of gentamicin(GM) and promote tubular regeneration when used after onset of acute renal failure.Methods:Thirtytwo male Wistar rats were allocate into three groups: Group I in which normal saline was given intraperitoneally for seven days,Group II and Group III in which GM was injected intraperitoneally for seven days separately.In the following seven days,rats in Group II drank freely as a model group,while animals in Group III were fed GS by gavage.All rats were sacrificed at the 15th day.Results:Serum creatnine(SCR),serum malondialdehyde(SMDA) and renal cortical malondialdehyde(RCMDA) levels in Group II were shown significantly higher than those in Group I and Group III.Serum superoxide dismutase(SSOD) and renal cortical superoxide dismutase(RCSOD) activities decreased statistically in Group II than those in Group I and Group III.Although the increment of SCR was not significantly higher in Group III compared to that in Group I,the increment of SMDA and RCMDA together with the decrement of SSOD and RCSOD activities was statistically different between them.And in comparison of that in Group II,light microscopic findings showed a mild damage and a rapid recovery of proximal tubular epithelial cells in Group III.Conclusion: Increment of reactive oxygen metabolites(ROM) and decrement of renal antioxidant ability play a role in GM nephrotoxicity in rats, and GS may ameliorate renal damage and promote recovery of proximal tubular cells and renal function via scavenging of ROM.
出处
《中日友好医院学报》
1998年第3期195-198,共4页
Journal of China-Japan Friendship Hospital
基金
卫生部科学基金
