摘要
研究了溶剂种类、浓度,提取溶剂比例、提取次数、时间、温度,萃取次数与温度,以及柱纯化和重结晶等条件对提取农杆菌中辅酶Q10的影响;进行了最佳工艺优化,确立了生产工艺,并进行了中试验证。结果表明,最佳工艺条件为:用5倍的体积分数95%的乙醇在50℃浸提2遍,每遍1 h,获得提取液;提取液浓缩后经过提取液-甲醇-石油醚(三者体积比为1∶2∶3)的萃取体系室温萃取3遍,浓缩石油醚相后经过1步硅胶层析柱纯化,2或3步乙醇重结晶。中试结果表明,该工艺获得辅酶Q10的纯度>99%,平均回收率>80%。
The process parameters of extraction CoQ10 from Agrobacterium were carefully investigated and then validated by pilot-scale test in this manuscript. The optimum process parameters were decided as follows： firstly, extracting twice （one hour each time） using 5X 95% enthanol at 50 ℃ followed by a concentrated step then extracting three times at room temperature using a extraction system ratio of 1 ： 2 ： 3 （concentrated solution ： methanol ： Petroleum ether） and finally concentrating Petroleum ether phase, through one step of Silica Gel-Alumina Column purification, two to three steps of recrystalization by enthanol to obtain the aim product. The pilot-scale test shown the process gives a purity of over 99%, percentage recovery over 80%.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第6期858-861,共4页
Journal of Food Science and Biotechnology
关键词
辅酶Q10
农杆菌
提取工艺
中试
Coenzyme Q10, Agrobacterium, Extracting, industrial production
