摘要
目的:研究在柱水解法自动化合成2-18F-2-脱氧-D-葡萄糖(2-[18F]-fluoro-2-deoxy-D-glu-cose,18F-FDG)。方法:采用在柱水解法和TRACERlabFXF-N自动化合成仪,以三氟甘露糖为前体,经亲核氟化、氢氧化钠在柱水解两步反应及SEP-PAK小柱分离纯化制备18F-FDG注射液。结果:18F-FDG总合成时间小于20min,未校正放化产率大于60%,放化纯度大于99%。结论:在柱水解法合成18F-FDG注射液,操作简便,是很实用的18F-FDG生产方法。合成的18F-FDG注射液可用于临床PET显像研究。
Objective To study automated synthesis of 2- [^18 F ] -fluoro-2 -deoxy-D-glucose （^18 F-FDG） via on-column hydrolysis. Methods Automated synthesis of ^18 F-FDG was performed by the on-column hydrolysis procedure in TRACERIab FXF-N synthesizer, ^18F-FDG injection was obtained via nucleophilic fluorination of 1, 3, 4, 6-tetra-O-acetyl-2-O-trifluoromethanesulfony-β-D-mannopyranose as the precursor molecule with ^18F-fluoride, hydrolysis of the ^18F-labeled intermediate on SEP-PAK C18 cartridges with 2 mol/L NaOH solution, and purification and neutralization with SEP-PAK cartridges. Results The uncorrected radiochemical yield of ^18F-FDG was more than 60% within the total synthesis time shorter than 20 min. The radiochemical purity of ^18F-FDG was above 99%. Conclusion On-column hydrolysis is simple and practical for the automated synthesis of ^18F-FDG. ^18F-FDG injection produced by this procedure can be used in clinical PET imaging.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2009年第11期1151-1156,共6页
Journal of Central South University ：Medical Science
