摘要
为探讨聚合酶链反应（ＰＣＲ）检测间日疟原虫（Ｐ．ｖ．）的现场应用价值。根据Ｐ．ｖ．红内期ＳＳｕｒＲＮＡ基因序列合成１对寡核苷酸引物建立检测Ｐ．ｖ．的ＰＣＲ技术，扩增产物片段为３４１ｂｐ，同时采用ＰＣＲ法和镜检法对７８份临床疑为Ｐ．ｖ．感染患者的血液标本进行检测。用ＰＣＲ法检测阳性者６９份，镜检法检测阳性者５９份，ＰＣＲ检出率（８８．５％）明显高于镜检法检出率（７５．６％），两者符合率为８７．２％。结果表明ＰＣＲ检测Ｐ．ｖ．具有简便、快速、特异性强、敏感性高等优点，可作为Ｐ．ｖ．
To explore field application value of detecting Plasmodium vivax(P.v.) by polymerase chain reaction (PCR),we synthesized a pair of oligonucleotide primers based on the sequence of the SSU rRNA gene in erythrocytic stages of P.v. and established the PCR method. The PCR product was a 341 bp fragment. Seventy eight blood samples from subjects suspiciously infected with P.v. were examined by PCR and microscopy. Of these, 69 were positive by PCR and 59 were positive by microscopy. The positive rate of the PCR(88.5%) was significantly higher than that of microscopy method(75.6%). The concordance rate between these two diagnostic methods was 87.2%. The PCR assay developed is a relatively simple, highly sensitive and specific method for detection of P.v. and can serve as a valuable tool for clinical diagnosis and epidemiological survey.
出处
《中国寄生虫病防治杂志》
CSCD
1998年第3期168-170,共3页
Chinese Journal of Parasitic Disease Control
关键词
间日疟原虫
聚合酶链反应
现场应用
Plasmodium vivax, polymerase chain reaction, field application
