摘要
目的:动物标本取材是变应性气道炎症研究中的重要实验步骤,文中探讨变应性气道炎症实验动物取材方法。方法:以豚鼠(20只)、大鼠(10只)和小鼠(20只)作为实验研究对象,分别行气管切开,经气管行鼻腔及肺部盥洗;其后对豚鼠和大鼠采用经心脏取血,对小鼠采用经心脏和眼部联合取血;最后采用不同手法取出鼻腔及肺部组织。结果:实验动物的各类标本均取材满意,鼻、肺盥洗液回收率为80%,大鼠、豚鼠血液平均收集量为2.5ml,小鼠平均收集量为1.5ml。结论:对变应性气道炎症鼠类动物模型的取材应根据种类的不同选用适合的操作手段和方法。
Objective： Sampledrawing is an important procedure in the study of allergic airway inflammation. The authors investigate the methods of drawing samples from the animals with allergic airway inflammation. Methods： We included in this study 20 Guinea pigs, 10 rats and 20 mice, which underwent trachea incision, followed by bronchoalveolar and rhinal lavage and collection of the lavage fluids. Then we collected blood samples via the heart from the guinea pigs and rats and via both the heart and the eyes from the mice, and obtained the tissues of the nasal cavities and lungs by different methods. Results ： All the samples were satisfactorily obtained from the animals, and 80% of the bronchoalveolar and rhinal lavage fluids were collected. Conclusion： Different methods should be adopted to suit different sampledrawing from the animal models of allergic airway inflammation.
出处
《医学研究生学报》
CAS
2009年第11期1147-1149,共3页
Journal of Medical Postgraduates
基金
江苏省博士后基金资助项目(批准号:0801030B)
关键词
变应炎症
气道
标本取材
Allergic inflammation
Airway
Sampledrawing
