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重组Psilencer1.0-U6-siRNA-stat3质粒对体外培养的大鼠主动脉平滑肌细胞的影响 被引量：1

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摘要 目的观察重组Psilencer1.0-U6-siRNA-stat3质粒对大鼠主动脉平滑肌细胞(RSMCs)的抗增殖作用机制。方法应用组织块方法培养RSMCs,应用脂质体转染方法将重组Psilencer1.0-U6-siRNA-stat3质粒转染体外培养的RSMCs,利用相差显微镜、MTT比色法观察体外培养的RSMCs增殖情况。结果培养72h后,相差显微镜下观察可见重组质粒组细胞增殖明显减少,MTT检测可见重组质粒组RSMCs的抑制率明显高于对照组。结论重组Psilencer1.0-U6-siRNA-stat3质粒能够抑制RSMCs增殖。
作者 孙江滨 赵科研 李博 吴慧颖 柳克祥
机构地区 牡丹江心血管病医院 吉林大学第二医院心外科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2009年第22期2921-2922,共2页 Chinese Journal of Gerontology
关键词 主动脉平滑肌细胞 STAT3 重组Psilencer1.0-U6-siRNA-stat3质粒
分类号 R3 [医药卫生—基础医学]
  • 相关文献

参考文献7

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共引文献1

同被引文献13

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中国老年学杂志
2009年 第22期

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