摘要
目的:探讨低氧和SD大鼠阴茎海绵体平滑肌细胞(CCSMC)凋亡的关系。方法:体外培养CCSMC,免疫组化鉴定细胞;低氧(1%O2浓度)干预12、24、48、72 h,常规氧浓度作为对照,流式细胞术测定细胞周期变化和凋亡情况。结果:体外培养的CCSMC生长良好,抗平滑肌α-肌动蛋白单克隆抗体免疫组化DAB法染色阳性。流式细胞术检测CCSMC G0/G1期在48 h内细胞比例逐渐增加,后下降;S期细胞比例与G0/G1期呈相反趋势;G2/M期细胞比例无明显规律。结论:CCSMC在低氧环境下,随时间的延长凋亡程度加重,48 h达到最大值,进一步延长时间细胞裂解,不能加重凋亡。
Objective： To explore the relationship between hypoxia and the apoptosis of corpus cavernosum smooth muscle cells（CCSMC） in SD rats.Methods： CCSMCs were cultured in vitro and identified by immunohistochemistry,and then underwent hypoxia interference at the concentration of 1%O2 for 12,24,48 and 72 hours,with normal oxygen concentration as the control.Flow cytometry was used to determine the cycles and apoptosis of the cells.Results： The cultured CCSMCs grew well,positive for anti-smooth muscle α-actin monoclonal antibody immunohistochemical staining.Flow cytometry showed that the number of CCSMCs in G0/G1 was gradually increased within 48 hours and then decreased,just opposite to the proportion of the S phase cells.But no regular change was found in the proportion of the cells in the G2/M phase.Conclusion： Hypoxia promotes the apoptosis of CCSMCs in a time-dependent manner,to the maximum at 48 hours,and then cell lysis may occur,but with no further apoptosis.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第11期990-993,共4页
National Journal of Andrology
基金
浙江省中医药科技计划项目(2005C038)~~
关键词
阴茎海绵体平滑肌细胞
低氧
凋亡
大鼠
corpus cavernosum smooth muscle cell
apoptosis
hypoxia
rat
