摘要
【目的】研究一氧化氮(NO)对绿豆侧根发生的影响。【方法】以绿豆为材料,用硝普钠(SNP)、NO特异清除剂c-PTIO和动物NOS抑制剂L-NAME单独处理绿豆侧根或与IBA、NAA混合处理,分析内源性NO对绿豆侧根发生的作用。【结果】SNP能显著促进绿豆侧根发生,其中以50μmol/L SNP处理24 h效果最佳,平均单株生根数为18;IBA或NAA与SNP混合处理的生根效果优于各自单独处理。L-NAME和c-PTIO单独处理或分别与IBA和NAA混合处理,均能抑制或延缓IBA和NAA诱导的侧根发生。【结论】内源NO在侧根发生及其形成中可能起着重要作用。
[Objective] Effect of nitric oxide（NO）on lateral rooting of mung bean was investigated. [Method] With mung bean as study material,the radicles were treated with sodicem nitroprusside（SNP）, c PTIO of NO specific scavenger and NOS inhibitor L-NAME alone or plus IBA or NAA to analyze the effect of endogenous NO on lateral rooting of mung bean. [Result] The results showed that SNP could significantly enhance the lateral rooting and the effects of IBA or NAA in stimulating rooting,50μmol/L SNP for 24 h being the best,average lateral roots per plant 18. c-PTIO of NO specific scavenger,or NOS inhibitor L-NAME treated alone or plus IBA or NAA resulted in an inhibitory effect. [Conclusion] It＇s indicated that endogenous NO appears to play a key role in the generation and development of lateral roots.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第12期119-122,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省自然科学研究计划项目(99SM20)
