摘要
目的:研究神经生长因子对体外培养的人卵巢组织雌孕激素分泌及KL mRNA表达的影响。方法:将10例人卵巢皮质切成薄片分成5组:未培养组、基础培养组、NGF组、抗NGF组及同型抗体对照组,培养结束后行雌孕激素测定及RT-PCR检测KL mRNA表达变化。结果:NGF组的KL mRNA表达0.91±0.30,略高于基础培养组的0.83±0.32,差异无统计学意义(P>0.05)。NGF组5个不同时间点的雌激素水平均高于其余各组,各组孕激素水平比较无统计学差异。结论:NGF能促进体外培养的人卵巢组织雌激素的分泌。
Objective： To investigate the effects of nerve growth factor （NGF） on hormone secretion of human ovarian tissue and expression of KL mRNA in vitro. Methods： Ovary cortex of 10 patients were cut into ovarian pieces and divided into 5 groups： non - culture group, basic culture group, NGF group, anti - NGF antibody group and homotype antibody group, after cultivation, the levels of estradiol and progestogen were detected, and the expression of KL mRNA was measured by RT - PCR. Results ： There was no difference in KL mRNA expression between NGF group （0. 91 ±0. 30） and basic culture group （0. 83±0. 32） （P 〉0. 05） . The levels of estradiol in 5 different time points in NGF group were significantly higher than those in the other groups, but there was no significant difference in progestogen level among the five groups. Conclusion： NGF can promote the secretion of estradiol of human ovarian tissues in vitro culture.
出处
《中国妇幼保健》
CAS
北大核心
2009年第35期5054-5056,共3页
Maternal and Child Health Care of China
基金
卫生部临床学科重点项目〔2004年〕
广州市卫生局重点科研项目〔2004Z1-E0101〕
广东省自然科学基金〔7001591〕
关键词
卵巢组织
原始卯泡体
培养神经
生长因子
Ovarian tissue
Primordial follicle
In vitro culture
Nerve growth factor
