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表达猪圆环病毒2型Cap和猪繁殖与呼吸综合征病毒GP5融合蛋白重组腺病毒的构建和免疫原性测定 被引量：3

Construction and immunogencity of recombinant adenoviruses expressing Cap protein of PCV2 and GP5 protein of PRRSV in mice
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摘要 断奶后多系统衰弱综合征(PMWS)是目前规模化猪场常见的传染病,由PCV2感染引起。临床上PCV2与PRRSV混合感染较常见,死亡率和发病率很高,但目前尚无能有效预防该病的商品化疫苗。本研究利用基因克隆方法,构建融合表达PCV2-Cap蛋白与PRRSV-GP5蛋白的重组腺病毒rAd-Cap-GP5,经IPMA、IFA和Western blotting验证了Cap蛋白和GP5蛋白在该重组病毒中获得表达。将该重组腺病毒免疫小鼠测定其抗体、中和抗体,结果显示小鼠免疫后出现两种病毒的抗体和中和抗体,诱导小鼠产生明显的体液免疫应答,可以作为预防PCV2-PRRSV混合感染的基因工程疫苗候选毒株,为PCV2和PRRSV二联重组亚单位疫苗的研究奠定了基础。 Porcine circovirus 2（PCV2） has been implicated as the etiological agent of postweaning multisystemic wasting syndrome（PMWS）.Co-infection of PCV2 and porcine reproductive and respiratory syndrome virus（PRRSV） can result in severe economic losses to the swine industry.In this study,we constructed the recombinant adenovirus rAd-Cap-GP5 expressing Cap of PCV2 and GP5 of PRRSV.And the expression of Cap and GP5 protein in the HEK-293 cells inoculated with rAd-Cap-GP5 were confirmed by immunoperoxidase monolayer assay（IPMA）,indirect immunofluorescence assay（IFA） and Western blotting,respectively.The immunogenicity of recombinant adenoviruses rAd-Cap-GP5 was examined in mice by vaccination with the recombinant adenovirus.The results showed that the mice could produce anti-PCV2 and PRRSV antibodies detected by indirect ELISA and virus neutralization assay.It indicated that rAd-Cap-GP5 could provide humoral immunity responses in mice.The recombinant adenovirus rAd-Cap-GP5 might be an attractive candidate vaccine for preventing the disease associated with PCV2 and PRRSV infection.
作者 王先炜 李玉峰 姜平
机构地区 南京农业大学农业部动物疫病诊断与免疫重点开放实验室
出处 《生物工程学报》 CAS CSCD 北大核心 2009年第11期1639-1645,共7页 Chinese Journal of Biotechnology
基金 国家自然科学基金(No.30871868) 国家科技支撑计划(Nos.2007BAD86B02-3 2006BAD06A04) 江苏省科技攻关项目(No.BE2007342) 教育部博士点基金项目(No.20060307007)资助~~
关键词 猪圆环病毒2型 猪繁殖与呼吸综合征病毒 重组腺病毒 免疫原性 PCV2 procine reproductive and respiratory syndrome virus recombinant adenovirus immunogencity
分类号 S852.5 [农业科学—基础兽医学]
  • 相关文献

参考文献17

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二级参考文献12

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共引文献30

同被引文献36

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引证文献3

二级引证文献4

生物工程学报
2009年 第11期

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