摘要
目的构建一个可靠有效的移植毛囊细胞诱导毛发发育的模型,以治疗脱发。方法取自愿捐献的成人头皮标本,联用显微分离与免疫磁珠法获得人毛囊干细胞;消化法获得毛乳头细胞。培养后混合植入裸鼠皮下,观察毛囊形成情况。结果在裸鼠的皮肤切片中可以看到较为完整的毛囊结构形成。结论毛囊细胞移植法可以在体内诱导出毛囊样结构,为将来治疗脱发奠定了基础。
Objective To establish a convenient and reliable method for inducing hair regeneration by follicular cell implantation for the treatment of alopecia. Methods The human hair follicle stem cells were separated and purified by micromanipulation and magnetic cell sorting, and human scalp dermal papilla cells were isolated by enzyme digestion. The two cells were mixed and implanted subcutaneously in nude mice to observe the regeneration of the hair follicles. Results Formation of intact hair follicle-like structures was observed in the skin sections of the recipient nude mice. Conclusion Follicular cell implantation can induce hair follicle-like structures in nude mice, which provides a means for efficient hair regeneration for treatment of hair loss.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第10期1987-1989,1993,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30872692)
广东省自然科学基金(8151051501000037)
广州市科技计划项目(2007Z3-E0021)
关键词
细胞疗法
毛囊干细胞
毛乳头细胞
毛发再生
cell therapy
hair follicle stem cells
dermal papilla cells
hair regeneration
