摘要
目的探讨多发性骨髓瘤(MM)常见染色体异常。方法结合1q21/RB1、D13S319/p53、IGH一组DNA特异性探针和荧光原位杂交(FISH)技术检测MM患者13q14缺失、+1、p53缺失以及IgH基因重排的发生率。结果22例MM患者中,13q14缺失7例(31.8%),其中D13S319、RB1探针同时检出4例13号染色体异常;IgH基因重排6例(27.2%);+113例(59.1%);p53基因缺失4例(18.1%)。不同免疫球蛋白类型的D13S319缺失率差异有统计学意义(P<0.05)。结论13q14缺失、IgH基因重排及+1在MM中发生率较高,FISH技术检测分子遗传学异常敏感度高,应作为MM的常规检查方法。
Objective To explore the chromosome abnormalities in multiple myeloma （MM）. Methods To combine a panel probes of LSI 1q21/RB1 , D13S319/p53, IGH and interphase flurorescenee in situ hybridization （FISH） were used to detect the 13q14 deletion,trisomy 1,p53 deletion and IgH gene rearrangements. Results Among 22 MM patients, 13q14 deletion was observed in 7（31.8% ）cases, the probe of D13S319 and RB1 were simultaneously 4 cases 13q14 deletion; 14q32 translocation was observed in 6（27.2% ）cases; trisomy 1 was observed in 13 （59. 1% ）cases; p53 deletion was observed in 4 （18. 1% ） cases;different types of immunoglobulin were associated with D13s319 deletion（P 〈 0. 05）. Conclusion The incidence of 13q14 deletion,lgH gene trans- location and trisomy 1 in multiple myeloma are high. FISH technique is a highly sensitive technique at detecting mo- lecular cytogenetic aberrations and shall be used in the routine evaluation of MM.
出处
《安徽医科大学学报》
CAS
北大核心
2009年第6期722-725,共4页
Acta Universitatis Medicinalis Anhui
基金
国家卫生部科研基金(编号:WKJ2007-3-001)
关键词
多发性骨髓瘤/遗传学
原位杂交
荧光
染色体畸变
multiple myeloma/genetics
in situ hybridization, flurorescenee
chromosome aberrations
