摘要
目的观察醛固酮拮抗剂——螺内酯对高糖诱导的大鼠肾小球系膜细胞纤溶酶原激活物抑制物-1(PAI-1)表达的影响。方法采用正常糖(NG)、高糖(HG)及HG+不同浓度螺内酯体外培养大鼠系膜细胞。以RT-PCR检测PAI-1、醛固酮受体(MR)和保护其配体特异性的11β-羟类固醇脱氢酶2(11β-HSD2)的mRNA表达,采用ELISA检测培养细胞上清中PAI-1蛋白的浓度。结果肾小球系膜细胞表达MR和11β-HSD2 mRNA;高糖刺激系膜细胞后PAI-1mR-NA表达明显升高,培养上清液蛋白浓度增加,加入螺内酯干预后系膜细胞PAI-1mRNA和蛋白表达明显下降,且随螺内酯浓度增大,作用更明显。结论螺内酯可能通过降低高糖对系膜细胞PAI-1表达的刺激作用而发挥其肾脏保护作用。
Objective To investigate the role of spironolactone on high glucose-induced plasminogen activator inhibitor-1 （PAI-1）expression of rat glomerular mesangial cells. Methods The rat mesangial cells were cultured in the medium with normal glucose（5.6 mmoL/L）, high glucose（25.0 retool/L） and high glucose add different concentrations of spironolactone（ 10-7,10-8,10-9 mmoL/L）. RT-PCR was used to detect the expression of PAI-1 ,min- eralocorticoid receptor （MR） and 11β-hydroxysteroid dehydrogenase （ 11β-HSD2） mRNAs. The level of PAI-1 in the supernatants was determined by ELISA. Results MR and 11β-HSD2 mRNA expressions were detectable in glomerular mesangial cells. Compared with normal glucose group, PAI-1 mRNA expression of mesangial cells and PAI-1 level in the supernatants increased significantly in high glucose group, while spironolactone inhibited those changes in a dose-dependent manner. Conclusion Spironolactone may play its reno-protection role via attenuating high glucose-induced PAI-1 expression of glomerular mesangial cells partly.
出处
《安徽医科大学学报》
CAS
北大核心
2009年第6期677-680,共4页
Acta Universitatis Medicinalis Anhui
基金
2008年安徽省国际合作项目基金(编号:2009GH043)
关键词
纤溶酶原激活物抑制物1
螺内酯
糖尿病肾病
plasminogen activator inhibitor 1
spironolactone
diabetic nephropathies
