摘要
[目的]研究剑麻花粉发芽率,为杂交授粉提供参考。[方法]以剑麻品种、蔗糖、琼脂、硼酸为因素,以花粉发芽率为统计指标,每个因素设3个水平,采用L9(34)正交设计。[结果]各因素对剑麻花粉发芽率的影响由大到小依次为品种、蔗糖、硼酸、琼脂;其中品种和蔗糖对剑麻花粉发芽率的影响极显著。[结论]剑麻花粉发芽率的最佳组合为A3B2C2D1,即H.11648在培养基组分为20%蔗糖+2.5%琼脂+0.010%硼酸时花粉发芽率最高。
[Objective] The aim was to study pollen germination rate of Agave sisalana,and provide reference for croas-pollination. [Method] Three levels of each factor were set up with Agave sisalana varieties,sugar,agar and boric acid as factors, orthogonal design was used with pollen germination rate as statistical indicators. [ Result ] The study showed that the effect of various factors on the pollen germination rate of Agave sisalana was varieties 〉 sucrose 〉 boric acid 〉 agar, of which the effects of varieties and sucrose on pollen germination rate of Agave sisalana were very significant. [ Conclusion] The best combination of pollen germination rate of Agave sisalana was A3B2C2D1 ,the pollen germination rate of H. 11648 was highest in 20% sucrose ＋2.5% agar ＋0.01% boric aeid.
出处
《安徽农业科学》
CAS
北大核心
2009年第34期16916-16916,17020,共2页
Journal of Anhui Agricultural Sciences
基金
现代农业产业技术体系建设专项(nycytx-19-E02)
中国热带农业科学院南亚热带作物研究所中央级公益性科研院所基本科研业务费专项(sscri200802)
2007年公益性行业(农业)科研专项(nyzx07-018)
关键词
剑麻
花粉
发芽率
Agave sisalana
Pollen
Germination rate
