期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MRSA的随机扩增多态DNA分型研究 被引量：2

暂未订购
导出
摘要 目的:应用随机扩增多态性DNA技术(RAPD),对耐甲氧西林金黄色葡萄球菌(MRSA)进行基因分型研究,以确定医院内是否存在MRSA流行。方法:收集我院临床分离的40株MRSA,采用RAPD技术进行同源性分析。结果:40株MRSA经电泳可得到1～11条片段,分为6个基因型。结论:RAPD分型技术简便、快速,在医院感染流行病学调查中具有较大应用价值。
作者 吴晓宁 覃锦耀 周元圆 韦惠如
机构地区 广西医科大学第一附属医院临床医学实验部 广西医科大学
出处 《广西医科大学学报》 CAS 2009年第5期731-732,共2页 Journal of Guangxi Medical University
关键词 MRSA RAPD 基因分型
分类号 R378.11 [医药卫生—病原生物学]
  • 相关文献

参考文献7

  • 1张庆玲,刘明华,王仙园.耐甲氧西林金黄色葡萄球菌的研究进展[J].中华医院感染学杂志,2002,12(10):797-798. 被引量：51
  • 2Sakoulas G , Gold HS , Venkataraman L, et al. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecApositive susceptible strains[J].J icrobiol Clin, 2001,39 (11):3 946- 3 951.
  • 3Tambic A, Power EG. Analysis of an outbreak of non pbage Typeable methicillin resistant Staphylococcus au reus hy using a randomly amplified polymorph DNA as say[J].J Clin Microbiol, 1997,35:3 092 -3 097.
  • 4Airesde Sousa M. Hde Lencastre. Bridges from hospi tals to the laboratory:genetic Portraits of methicillin-resistant Staphylococcus aureus clones[J]. FEMS Immunol Med Microbiol.2004,40:101 -111.
  • 5Hardy KJ. Ussery DW, Oppenheim BA, et al. Distributton and characterization of Staphylococcus inter spersed repeat units (SIRUs) and polential use for strain differentiation [J].Microbiology, 2004, 150 (Pt12):4 015- 4 052.
  • 6张亚莉,周宏伟,马骊,温茜,王茵茵,李琼,汪能平.医患鼻前庭携带耐甲氧西林金黄色葡萄球菌与医院感染菌同源性的研究[J].南方医科大学学报,2006,26(5):635-637. 被引量：6
  • 7孙宏莉,王辉,陈民钧.甲氧西林耐药的金黄色葡萄球菌耐药性及分子流行病学调查[J].中华微生物学和免疫学杂志,2005,25(2):137-141. 被引量：61

二级参考文献22

  • 1舒雪芹,方晔,石娜,周铁丽,廖洪菊.医院环境中葡萄球菌监测[J].中华医院感染学杂志,2004,14(7):771-772. 被引量：11
  • 2苏关关,俞云松,林洁,俞美红,马亦林.19株甲氧西林耐药金黄色葡萄球菌的同源性分析[J].中华流行病学杂志,2004,25(12):1084-1085. 被引量：2
  • 3孙宏莉,王辉,陈民钧.甲氧西林耐药的金黄色葡萄球菌耐药性及分子流行病学调查[J].中华微生物学和免疫学杂志,2005,25(2):137-141. 被引量：61
  • 4Jevons MP. "Celbenin"-resistant Staphylococci. Br Med J,1961,1: 124-125.
  • 5Barberis-Maino L,Berger-Bachi B,Weber H,et al. IS431,a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgaris. Gene,1987,59: 107-113.
  • 6NCCLS. Performance standards for antimicrobial susceptibility testing; thirteenth informational supplement. NCCLS,2003,M100-S13.
  • 7Jonas D,Grundmann H,Hartung D,et al. Evaluation of the mecA femB duplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis,1999,18: 643-647.
  • 8Jonas D,Speck M,Daschner FD,et al. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. J Clin Microbiol,2002,40: 1821-1823.
  • 9Tenover FC,Areit RD,Goering RV,et al. Interpreting chromosomal DNA restriction patterns produced by pulse-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol,1995,33: 2233-2239.
  • 10Chambers HF,Neu HC. Principles and practice of infectious diseases. Penicillins,1995,6:233-246.

共引文献115

同被引文献20

  • 1Elina R,Susana B,Cecilia F,et al. RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts [ J ] . Microbiol Res, 2004,159: 245-255.
  • 2Neela V,Mariana NS,Radu S,et al. Use of RAPD to investigate the epidemiology of Staphylococcus aureus infection in Malaysian hospitals [ J ]. World J Microbiol & Biotechnol, 2005,21 : 245-251.
  • 3Randa GN,Salwa M,Bdour HM,et al . Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan [J] . J Med Microbiol, 2006,55 : 183-187.
  • 4Wang L,Barrett J F. Control and prevention of MRSA infections [J]. Methods Mol Biol, 2007,391 : 209-225.
  • 5Deurenberg RH,Kalenic CVS,A FRIEDRICH W,et al. The molecular evolution of methicillin-resistant Staphylococcus aureus[J ]. Clin Microbiol Infect, 2007,13 : 222-235.
  • 6Bou G,Cervero G,Domingucz MA,et al . PCR-based DNA fingerprinting REP -PCR,AP -PCR and pulsedfield gel electrophoresis characterization of a nosocomial outbreak caused by imipenem and meropenem-resistant Acinetobacter baumannii [J] . Clin Microbial Infect, 2000,6 : 635-643.
  • 7Sabour P M,Gill J J,Lepp D,et al . Molecular Typing and Distribution of Staphylococcus aureus isolates in Eastern Canadian Dairy Herds [J] . J Clin Microbiol,2004,42(8):3449-3455.
  • 8Chioc, Weih, Yang L. Comparison of pulsed-field gelelectrophoresis and coagulase gent restriction profile analysis techniques in the molecular typing of Staphylococcus aureus [J] . J Clin Microbiol, 2000,38: 2186-2190.
  • 9Airesde SM,H de leneaster . Bridges from hospitals to the laboratory:genetic Portraits of methicillin-resistant Staphylococcus aureus clones [J] . FEMS Immunol Med Microbiol,2004,40:101-111.
  • 10Hardy KJ,Ussery DW,Oppenheim BA,et al . Distribution and characterization of Staphylococcus interspersed repeat units (SIRUs) and potential use for strain differentiation [J] . Microbiology, 2004, 150( 12 ) : 4045-4052.

引证文献2

二级引证文献8

广西医科大学学报
2009年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部