摘要
利用PCR-PstⅠ-RFLP技术对德化黑鸡的胰岛素样生长因子-1(IGF-1)基因的5′端调控区进行多态性分析,并探讨了多态性与产蛋性状的相关性。结果表明,该调控区的扩增产物用PstⅠ酶切检测出3种基因型AA、AB、BB。利用最小二乘分析研究了该多态位点对产蛋性状的影响,结果表明该多态位点对开产日龄没有显著影响,对240日龄产蛋数和300日龄产蛋数有显著影响(P<0.05)。其中AA基因型的240日龄产蛋数和300日龄产蛋数显著高于AB基因型和BB基因型,表现为差异显著(P<0.05),AB基因型的240日龄产蛋数和300日龄产蛋数高于BB基因型,但表现为差异不显著(P>0.05)。
The genetic polymorphism of 5′- regulatory region of IGF - 1 gene in Dehua black chicken was analyzed by PCR - Pst Ⅰ - RFLP. The relationship between the polymorphic locus and egg-laying was established using the least square analysis. There were three genotypes, AA, AB and BB, detected on the Pst Ⅰ site. The result showed that there was no significant difference in the age of the chickens when egg-laying started, but there was a significant difference in the number of eggs laid at 240 days and 300 days （P〈0.05）. The numbers of eggs at 240 days and 300 days for the chickens with the AA genotype was significantly higher than those with the AB or BB （P〈0. 05）. The number of eggs laid at 240 days and 300 days for the chickens with the AB genotype was higher than that with BB, but not significantly （P〈0.05）.
出处
《福建农业学报》
CAS
2009年第5期404-407,共4页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目(2007N0059)
关键词
德化黑鸡
IGF-1基因
多态
产蛋性状
Dehua black chick
IGF- 1
polymorphism
egg-laying performance
