摘要
利用原子力显微镜分别对未加药人急性T淋巴细胞性白血病细胞(Jurkat细胞)和经超抗原刺激不同时间(6,12,48,72h)后的Jurkat细胞进行了细胞全貌和细胞膜表面纳米结构成像和探测研究,并通过比较不同状态下细胞表面的粘附力变化,探讨Jurkat细胞形态变化与粘附行为之间的关系,用CCK-8检测细胞的增殖,以期对Jurkat细胞形态结构和细胞功能之间的关系有进一步的了解。结果发现:与未加药的Jurkat细胞相比,随着超抗原刺激时间的延长,Jurkat细胞的体积、高度、半宽度、粗糙度等参数发生明显的变化;活化48、72h时,细胞与针尖间的相互作用力大约是活化6h时的5倍,活化过程中细胞膜表面纳米结构的改变,引起其机械性能的变化。Jurkat细胞的表面超微结构、细胞膜结构的改变和分化对于更深入地了解T细胞活化与免疫信号的传递,阐明其免疫过程的作用机制具有重要的意义。
In this article, AFM was used to study the cellular surface ultrastructures and nanomechanical properties of acute T lymphoblastic leukemic cell （Jurkat cell） which was treated with staphylococcal enterotoxin A （SEA） at different time （6, 12, 48, 72 h）, and CCK-8 was used to detect the proliferation. The changes of adhesive force of Jurkat cell at different states and the relation between the changes of cellular morphology and the changes of cellular surface adhension, to get a more knowledge of the relation between morphostructure and cellular function. The result showed that the morphostructure and cell membrane of Jurkat cell exposed to SEA changed significantly and the ultrastructures became more complex with the time prolonging. The adhesion force values of Jurkat cells treated with SEA at 24 and 48 h were five times than those of Jurkat cell treated with SEA at 6 h. The changes of the ultrastructures and membrane structure of Jurkat cell have contributed to understand deeply T-cell activation and immune signal transmission. The changes have also played an important role in explaining mechanism of immunologic process.
出处
《生物物理学报》
CAS
CSCD
北大核心
2009年第5期373-380,共8页
Acta Biophysica Sinica
基金
国家自然科学基金项目(30872404)~~
关键词
原子力显微镜
超抗原
JURKAT细胞
Atomic force microscopy
Staphylococcal enterotoxin A （SEA）
Jurkat cell
