摘要
目的探讨肿瘤抑制基因ｐ１６在几种类型白血病中的改变及其频率。方法对７７例原发性白血病及非霍奇金淋巴瘤标本分别采用Ｓｏｕｔｈｅｒｎ印迹杂交，ＰＣＲ┐ＳＳＣＰ的方法检测缺失及点突变。结果Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交发现ｐ１６基因异常共１１例，其中纯合缺失７例，异常重组片段４例，ＳＳ┐ＣＰ发现ｐ１６第三外显子异常泳动带６例，是否为突变有待测序证实。结论ｐ１６基因改变主要集中在急性淋巴细胞性白血病，占４７．７％（９／１９）。
Objective:The purpose is to access the incidence of p16 gene alteration in several types of leukemias.Methods:Southern blot hybridization and PCR SSCP were used to detect deletion and mutation of p16 gene in 77 cases of hematologic malignancies respectively.Results:11 cases with p16 alteration were detected in Southern blot hybridization and 7 of them were homozygous deletion and 4 had abnormal recombinated fragement.Abnormal band migrations were detected in exon 3 and whether they are mutations or not require to be confirmed by direct sequencing analysis.Conclusion:The p16 alteration is a frequent event in leukemia and it is restricted to lymphoid neoplasm and especially to ALL. This result indicates that this tumor suppressor gene may play an important role in tumorgenesis.
出处
《肿瘤》
CAS
CSCD
北大核心
1998年第6期381-384,共4页
Tumor
