摘要
目的通过检测DNA-PKcs在肺腺癌细胞中的表达情况,探讨其与放射敏感性的关系。方法成克隆实验分别测定肺腺癌细胞株A549、H1299照射后的剂量-存活曲线,Western blotting及DNA-PK活性分析法检测两株细胞中DNA-PKcs的含量与活性。分析放射敏感性与DNA-PKcs的关系。结果成克隆实验结果显示:肺腺癌细胞H1299较A549放射更敏感,SF2分别为A549:0.7412,H1299:0.2473。Western-blot显示A549和H1299积分光密度比值分别为:3.29±0.44、0.50±0.17,A549中DNA-PKcs的表达更高(t=10.37,P<0.001)。DNA-PKcs活性检测结果为A549:8.29±1.37,H1299:2.47±1.09(t=5.76,P=0.005)。结论放射敏感性不同的肺腺癌细胞株中DNA-PKcs的表达不同,提示DNA-PKcs可能是影响肺腺癌细胞放射敏感性的重要因素。
Objective To investigate DNA-dependent protein kinase catalytic subunit（DNA-PKcs） content and activity in lung adenocarcinoma cell lines and its correlation with radiosensitivity.Methods The content and activity of DNA-PKcs were analyzed in two lung adenocarcinoma cell lines A549 and H1299 by Western blotting and the Signa TECT DNA-PK assay kit.The dose-survival relationship for two cell lines was analyzed using clonogenic formation assay.Results A549 was more radiosensitive than H1299.The survival fractions at 2 Gy（SF2） were 0.7412 in A549 cell line and 0.2473 in H1299 cell line.The content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells（t=10.37,P〈0.001）.The integrated optical densities were 3.29±0.44 in A549 cells and 0.50±0.17 in H1299 cells.DNA-PKcs activities in A549 and H1299 cells were 8.29±1.37 and 2.47±1.09,respectively,showing a significant difference between them（t=5.76,P=0.005）.Conclusion DNA-PKcs is an important factor to affect the radiosensitivity of lung adenocarcinoma cell lines.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第11期2241-2243,共3页
Journal of Southern Medical University
基金
广东省医学科学技术研究基金(A2006014)
广东省自然科学基金(06020905)
关键词
DNA-PKCS
肺腺癌
放射敏感性
DNA-dependent protein kinase catalytic subunit
adenocarcinoma
radiosensitivity
