摘要
目的观测大鼠局灶性脑缺血再灌注模型缺血侧纹状体区内白细胞介素－１β（ＩＬ－１β）和肿瘤细胞坏死因子－α（ＴＮＦ－α）的动态变化，及甲基强的松龙和榄香烯乳对其的影响。方法用线栓法阻塞大脑中动脉，用ＥＬＩＳＡ法检测ＩＬ－１β和ＴＮＦ－α。结果缺血１ｈ再灌注１ｈ后即有ＴＮＦ－α成倍升高，６ｈ达高峰，随后逐渐下降；ＩＬ－１β在再灌注３ｈ升高。缺血后即刻给予３ｍｇ／ｋｇ甲基强的松龙或１０ｍｇ／ｋｇ榄香烯乳可以降低ＴＮＦ－α水平，但不影响ＩＬ－１β水平。结论局灶性脑缺血再灌注后有ＩＬ－１β和ＴＮＦ－α的升高，６ｈ达高峰；３ｍｇ／ｋｇ甲基强的松龙或１０ｍｇ／ｋｇ榄香烯乳可以降低缺血区ＴＮＦ－α水平。
Objective We observed the kinetic changes of IL 1 β and TNF α in the ischemic striatum of focal ischemic reperfusion rats,and the effect of methylprednisolone or elemene emulsion on them.Methods The middle cerebral artery in rats was occluded by an intraluminal filameat.IL 1 β and TNF α were measured by ELISA methods.Results TNF α timely increased in 1 hour ischemia and 1 hour reperfusion and reached peak level in 6 hour reperfusion,and then gradually decreased.However IL 1 β only elevated after 3 hour reperfusion.Infusion of 3 mg/kg of methylprednisolone or 10 mg/kg elemene emulsion after ischemia could reduced the level of TNF α,but had no effect on IL 1 β.Conclusion Focal cerebral ischemic reperfusion in rats leaded to the increases of IL 1 β and TNF α in the ischemic striatum and reached peak level in 6 hour reperfusion.Infusion 3mg/kg of methylprednisolone or 10 mg/kg elemene emclsion could reduce the level of TNF α in the ischemic striatum.
出处
《中风与神经疾病杂志》
CSCD
北大核心
1998年第5期262-264,共3页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金
