摘要
【研究目的】对大花蕙兰品种‘绿宝石’与春剑杂交原球茎增殖与分化进行研究。【方法】设置不同培养基、6-BA和NAA以及不同添加物对杂交兰原球茎增殖分化的进行探讨。【结果】KC培养基为最佳培养基;6-BA对原球茎的增殖影响不大,NAA对增殖影响较明显当浓度为0.2 mg/L原球茎增殖效果最好;KC+6-BA2.0 mg/L+NAA 0.5 mg/L对原球茎的分化效果最好;【结论】在培养基中加15 ml/L香蕉汁最有利于杂交兰原球茎的增殖。
[OBJECTIVE]The research aimed to study the propagation and differentiation of PLB of Cymbidium hybridum＇Queen Emeraldus＇and C. longibracteatum. [METHOD] Four factors of medium, 6-BA, NAA, and ad- ditive were designed to study the effects on differentiation and differentiation of PLB of Cymbidium hybridum ~ C. longibracteatum. [RESULTS]KC medium was more effective than others. There was no difference with differ- ent concentrations of 6-BA. NAA influenced the propagation of PLB directly. NAA0.2 mg/L was most favorable to the propagation of PLB. The best differentiation medium was KC＋6-BA2.0 mg/L＋NAA0.5 mg/L. [CONCLU- SION] the medium supplemented with 15 ml/L banana was most beneficial to the Propagation of PLB.
出处
《中国农学通报》
CSCD
北大核心
2009年第23期327-330,共4页
Chinese Agricultural Science Bulletin
基金
泰安市大学生科技创新项目(2008D2012)
关键词
杂交兰
原球茎
增殖
分化
Cymbidium hybridumxC, longibracteatum, protocorm like-body, propagation, differentiation
