摘要
为了更好地了解水稻腈水解酶家族的功能,采用生物信息学的方法对水稻中的腈水解酶进行了一系列分析,结果发现水稻中共有8个编码腈水解酶的基因,虽然基因结构很不相同,但其所编码的腈水解酶都具有典型的碳氮水解酶结构域和保守的Glu-Lys-Cys催化三联体。通过EST搜索发现这些基因在水稻不同组织和不同胁迫条件下的表达情况存在较大的差异。此外,对水稻和拟南芥腈水解酶进行系统进化分析发现其分别处于5个不同的分支,表明它们的分化早于单子叶植物和双子叶植物的分化。研究结果为进一步研究该类蛋白的分子和生物学功能奠定了良好基础。
Nitrilases are important enzyme in plants. In order to understand the function of rice nitrilases, de- tailed analysis had been done by bioinformatics methods. Eight genes coding nitrilase were identified in the Oryza sativa L. ssp. japonica protein database. Structures of these genes were different, but all the proteins they coded had a typical carbon-nitrogen hydrolase domain and a conserved Glu-Lys-Cys catalytic triad. ESTs analysis showed that each gene expressed diversely in different tissues or under different stresses, which sug- gested specific function of particular gene. In addition, the phylogenic analysis showed that the nitrilases of rice and Arabidopsis were grouped in 5 clusters, suggesting that they evolved earlier than monocotyledon and dicotyledon. In a summary, these data will facilitate to further characterize molecular or biological functions of the proteins.
出处
《中国农学通报》
CSCD
北大核心
2009年第23期52-57,共6页
Chinese Agricultural Science Bulletin
基金
福建省自然科学基金"水稻广谱防御基因的克隆与功能分析"(2003F008)
