摘要
目的检测喉鳞状细胞癌(LSCC)中肿瘤抑制基因PTEN(与细胞骨架蛋白Tensin同源在肿瘤第10号染色体有缺失的磷酸酯酶)和胰岛素样生长因子1-受体(IGF-1R)蛋白的表达情况,并分析它们与LSCC临床病理因素的关系。方法取喉部鳞状细胞癌石蜡标本50例作为研究对象,30例声带息肉(VCP)黏膜标本作为对照组。采用免疫组织化学SP法测定PTEN和IGF-1R的表达,分析PTEN和IGF-1R的表达与LSCC的组织学分化程度、T分级、临床分期及淋巴结转移等的关系。统计学分析应用统计软件SPSS13.0进行分析。结果PTEN蛋白在LSCC、VCP阳性表达率分别为58.0%和96.7%,两者比较差异有显著统计学意义(P<0.01)。随LSCCT分期、临床分期的增加、分化程度降低及淋巴结转移,PTEN阳性表达率逐渐降低。IGF-1R蛋白在LSCC、VCP阳性表达率分别为68.0%和16.7%,两者比较,差异有显著统计学意义(P<0.01)。随LSCCT分期、临床分期的增加、分化程度降低及淋巴结转移,IGF-1R阳性表达率逐渐增高。PTEN表达与IGF-1R表达呈明显负相关。结论PTEN缺失和(或)蛋白的失活和IGF-1R蛋白的高表达可能是喉鳞癌的发病机制之一,PTEN与IGF-1R两者之间可能存在失衡,并可能在LSCC中起作用。
Objective To detect the expression of PTEN protein and IGF-1R protein in the laryngeal squamous cell carcinoma （LSCC）, and to analyze relationship with LSCC＇ clinicopathological factors. Methods To take 50 pieces of petroline samples of laryngeal squamous cell carcinoma as study object, to take 30 pieces of VCP mucosae samples as control group. To detect the expression of PTEN protein and IGF-1R protein in LSCC and VCP by S-P immunohistochemistry method, to analyze the relationship with histology differentiated degree, T grade, clinical stage and lymph node diversion of LSCC. Statistic analyses were done by SPSS 13.0 software. Results The positive rates of PTEN were respectively 58% ,96.7% in LSCC, and VCP, there were notable significant difference between them statistically （ P 〈 0. 01 ）. With increasing of T grade, clinical stage and lymph node diversion, the positive rates of PTEN were basically gradually cutting down. The positive rates of IGF-1R were respectively 68%, 16.70% in ISCC, and VCP, there were notable significant difference between them statistically （P 〈 0. 01 ）. With increasing of T grade, clinical stage and lymph node diversion, the positive rates of IGF-I R were basically gradually rising. PTEN expression was obviously negative correlation with IGF-1R expression. Conehmions Absence and/or deactivation of PTEN and overexpression of IGF-1R are probably section of LSCC＇ disease mechanism, there is probably disequilibrium between PTEN and IGF-1 R, and this disequilibrium probably play a role in LSCC.
出处
《中华临床医师杂志（电子版）》
CAS
2009年第11期43-48,共6页
Chinese Journal of Clinicians(Electronic Edition)
关键词
喉肿瘤
肿瘤
鳞状细胞
基因
肿瘤抑制
受体
IGF1型
肿瘤形成过程
Laryngeal neoflasms
Neoplasms, squamous cell
Genes, tumor suppressor
Receptor,IGF type 1
Neoplastic processes
