摘要
目的：探讨肝硬化时肠源性内毒素血症（ＩＥＴＭ）和血浆内皮素（ＥＴ）在肾功能障碍中所起的作用。方法：采用鲎试剂基质显色法定量测定内毒素，用放射免疫法测定ＥＴ，常规生化法测定血肌酐，同时留２４小时尿液测定尿肌酐与尿钠。采用复合病因复制肝硬化模型。结果：肝硬化患者血浆内毒素水平随肝硬化病变加重而升高，内毒素水平在肝硬化伴功能性肾衰患者中（２０６±１５１ＥＵ／ｍＬ）明显高于肝硬化代偿期（０３２±０２０ＥＵ／ｍＬ）与失代偿期伴腹水（０７３±０２９ＥＵ／ｍＬ），并与肌酐清除率（ＣｒＣｌ），尿钠排泄呈负相关。肝硬化鼠血浆内毒素与肾组织ＥＴ含量（分别０２８±００４ＥＵ／ｍＬ，４６２±０７９ｎｇ／ｇ）明显高于对照组（分别０１４±００２ＥＵ／ｍＬ，２４８±１８８ｎｇ／ｇ），两者呈正相关。结论：ＩＥＴＭ在诱发象征肝功衰竭的ＦＲＦ中起重要作用；
Abstract AIM:To study the roles of intestinal endotoxemia(IETM)and endothelin(ET) on renal dysfunction in cirrhosis.METHODS:The plasma endotoxin,endothelin,creatinine,and 24 hours urine creatinine and sodium were measured by Limulus test method,radioimmunological method and biochemical analysis,respectively.The cirrhotic model was developed using complex pathogeny.By the same methods,we also measured plasma endotoxin,endothelin and creatinine,and 16 hours urine creatinine and sodium of rats.The endothelin content in renal tissues was also measured.RESULTS:The plasma endotoxin level of cirrhotic patients was increased with severity of cirrhosis.The endotoxin level in cirrhosis with functional renal failure(2.06±1 51EU/mL)was singnificantly higher than that in compensated cirrhosis(0.32±0.20 EU/mL)and decompensated cirrhosis with ascites(0.73±0.29EU/mL),which was negatively correlated with creatinine clearance and urine sodium excretion.In cirrhotic rats,the plasma endotoxin level (0.28±0.04 EU/mL) and endothelin content in renal tissues(4.62±0.79 ng/g)were obviously higher than that of the control (0.14±0 02EU/mL and 2.48±1.88ng/g,respectively),which showed positive correlationship.CONCLUSION:Intestinal endotoxinemia plays and iimportant role in induciing functional renal faiure which is the symbol of hepatic failure.Intestinal endotoxinemia has certain effects on producing and releasing of endothelin in renal tissues. MeSH Live cirrhosis;Endotoxine;Endothelins;Kidney
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1998年第6期717-719,共3页
Chinese Journal of Pathophysiology
