摘要
目的：γδＴ细胞具有细胞毒效应，探讨γδＴ细胞体外活化的规律将有助于这类细胞的临床应用。方法：将成人外周血分别置于ＰＨＡ（４０ｍｇ／Ｌ）、ＰＤＢ（２×１０－７ｍｏｌ／Ｌ）及ＰＨＡ（４０ｍｇ／Ｌ）加上ＰＤＢ（２×１０－７ｍｏｌ／Ｌ）的条件下进行全血培养，在培养２ｈ和６ｈ后分别用双色免疫标记流式细胞法对外周血Ｔ细胞的ＴＣＲαβ、ＴＣＲγδ和ＣＤ６９分子进行分析。结果：Ｔ细胞在受刺激后２ｈＣＤ６９分子即有所增高，但其增加的程度因Ｔ细胞亚群和刺激剂不同而有差别，ＰＨＡ和ＰＤＢ分别使αβＴ细胞ＣＤ６９表达百分率由对照组的４４８±１３６升高到８７９±１６７和７３３６±２９８；ＰＨＡ和ＰＤＢ分别使γδＴ细胞ＣＤ６９表达百分率由对照组的１３６５±１７４升高到３５８４±１４０和９９０３±０９７。与２ｈ的情况相比，αβＴ细胞在各种刺激剂作用６ｈ后ＣＤ６９表达均有进一步的增高，γδＴ细胞在ＰＨＡ作用６ｈ后ＣＤ６９表达继续上调。结论：γδＴ细胞对多克隆活化剂的反应比αβＴ细胞更早和更强。这一特征更有利于体外扩增γδΤ细胞并将其用于临床免疫治疗。
Abstract AIM:To investigate the details of γδ T cell activation in vitro will be helpful for the application of these cells clinically. METHODS:The whole blood culture procedure was applied to mimic the natural conditions of T cell activation in vivo .T cells were activated by PHA(40mg/L),PDB(2×10 -7 mol/L) and PHA(40mg/L)+PDB(2×10 -7 mol/L) respectively.The expression rates of TCRαβ ,TCRγδ and CD69 of 2 and 6-hour-cultured lymphocytes were estimated by flow cytometer following two-color immunofluorescent staining.RESULTS:The expression rates(%) of CD69 began to increase after 2 hour stimulation,which were variant with T cell subsets and activators.The expression rates of CD69 of αβ Τ cells in response to PHA and PDB stimulation for 2 hours increased from 4 48±1 36 in control group to 8 79±1 67 and 73 36±2 98 respectively.The expression rates of CD69 of γδ T cells in response to PHA and PDB stimulation for 2 hours increased from 13 65±1 74 in control group to 35 84±1 40 and 99 03±0 97 respectively.A further increase of CD69 expression after 6 hour culture was seen in αβ T cells stimulated by PHA and PDB as compared to the results after 2 hour culture,for γδ T cells a further increase only can be seen in response to PHA.CONCLUSIONS:γδ T cells express CD69 in response to a variety of polyclonal activators was more rapidly and at a higher rate as compared to αβ T cells.This characteristic of γδ Τ cells will be more favorable to the in vitro expansion of γδ T cells for clinical application. MeSH T lymphocytes;Antigens,surface;T cell activation;Flow cytometry
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1998年第6期579-582,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
