摘要
应用温和热处理诱导人急性髓系白血病细胞系ＨＬ－６０细胞发生凋亡，观察到典型的细胞形态学改变和特征性的ＤＮＡ梯型；维甲酸可促使细胞凋亡，而重组人粒－巨噬系集落刺激因子的抑制作用轻微。用斑点杂交法检测温和热处理组及维甲酸加温和热处理组细胞的ｃ－ｍｙｃ基因ｍＲＮＡ表达水平。
The effects of several factors on the apoptosis of HL 60 cells, a human acute myeloid leukemia cell line, were investigated. The apoptosis model was established with mild heat. Typical changes of cell morphology and characteristic DNA ladder pattern were found. The effect of apoptosis induced by mild heat was enhanced, when HL 60 cells were pretreated with the retinoic acid. By contraries, that of apoptosis was displayed with the slight inhibition while HL 60 cells were pretreated with rhGM CSF. We also observed the alterations of mRNA expressions of c myc by the dot hybridization. The results indicate that both the mild heat and the combination of mild heat and retinoic acid obviously reduce the mRNA expression of c myc compared to the control group.
出处
《湖南医科大学学报》
CSCD
1998年第6期524-526,共3页
Bulletin of Hunan Medical University
基金
湖南省卫生厅基金
关键词
细胞凋亡
急性
白血病
HL-60细胞
HL 60 cells
cell death
retinoic acid
granulocyte macrophage colony stimulating factor
genes
