摘要
以7个大白菜杂交种为试材进行花药培养,分析了培养基pH、激素和氨基酸浓度对胚状体发生的影响,以及活性炭对胚状体成苗的促进作用。结果表明：以MS为基本培养基,其适宜的pH为5.6～6.1,激素浓度为NAA 2 mg/L＋6-BA 4 mg/L,脯氨酸浓度为4 mg/L;添加0.8%的活性炭有利于胚状体继代培养和成苗。
Anther culture was carried out by use of seven F1 hybrids of Chinese cabbage（Brassica campestris ssp.pekinensis）.Effects of medium pH value,hormone and amino acids concentration on embryoid inducing were probed,as well as activated carbon on plantlet forming.Results showed that suitable rang of medium pH value was 5.6～6.1;the appropriate hormone proportion was NAA 2 mg/L＋6-BA 4 mg/L;the appropriate amino acid proportion was proine 4 mg/L;0.8% activated carbon were benefit to the subculture medium.
出处
《北方园艺》
CAS
北大核心
2009年第11期71-73,共3页
Northern Horticulture
基金
国家公益性行业(农业)科研专项资助项目(nyhyzx07-007)
关键词
大白菜
花药培养
胚状体
再生植株
Chinese cabbage
Anther culture
Embryoid
Plantlet
