摘要
毒蛋白编码基因Bt886cry3Aa对小黄菊进行了遗传转化,转化植株经过PCR和总DNA狭缝杂交验证,得到转基因小黄菊4个样品株。对菊天牛2龄幼虫的生物测定表明:4个样品株毒杀活性明显高于对照,校正死亡率分别为:45.89%、39.12%、35.42%和22.41%。mBt886cry3a转基因植株表现出较强的天牛毒杀活性,为天牛等钻蛀性害虫的防治提供了研究基础。
Bt886Cry3Aa was cloned from Bacillus thuringiensis 886 strain,which was shown toxic to the larva of long horn beetle.Based on the characters of coding usage for pulpous genes,mBt886cry3a was artificially modified and then named mBt886cry3a.Anoplophora glabripennis infests Chrysanthemum Dendrathema grandiflorum Xiao Huang seriously in Henan province.Transgenic approach may be an efficient way to control long horn beetle.In this study, mBt886cry3a were transformed into Chrysanthemum Dendratkerna grandiflorum ‘Xiao Huang’ through Agrobacterium tumefaciens-mediated transformation. Four independent transformed lines of mBt886cry3a ly were obtained, by PCR and DNA blotting. Bioassay of four transgenic lines to second instars larvae of Anoplophora glabripennis under the control condition showed that they gave the mortalities of larvae from high as 45.89%, 39.12% and 35.42% to low as 22. 41%. There was a statistical difference between the transgenic lines and wide line （P〈0. 05）. The mBt886cry3a transgenic chrysanthemum plants exhibited high activity against Anoplophora glabripennis, thus this study provides the basis for the genetic modification on plant resistant to long horn beetles.
出处
《北方园艺》
CAS
北大核心
2009年第11期48-50,共3页
Northern Horticulture
基金
河南省杰出人才创新基金资助项目(074200510018)
河南科技大学博士科研启动基金资助项目(09001265)
关键词
菊天牛
毒蛋白
遗传转化
小黄菊
Phytoecia rufiventris
Bt
Agrobacterium tumefaciens-mediated transformation
Dendrathema grandiflorum Xiao Huang
