摘要
目的 探讨阿糖胞苷（Ara-C）对急性白血病（AL）细胞B7分子表达和激活T细胞的免疫反应。方法 用流式细胞术检测原代AL细胞B7分子的表达。同时检测Ara-C诱导HL-60细胞 B7分子的表达，进而用RT-PCR方法检测HL-60细胞B7 mRNA表达。MTT法检测诱导后HL-60细胞对人外周血单个核细胞（PBMC）的促增生作用，并用RT-PCR法测定γ-干扰素（IFN-γ）含量。结果 40例AL患者中B7-1阳性1例，B7-2阳性3例、弱阳性4例。Ara-C能显著刺激HL-60细胞 B7表达，诱导后的HL-60细胞显著刺激PBMC增生并产生IFN-γ。结论 原代AL细胞低表达B7-1和B7-2分子。在体外，Ara-C通过诱导HL-60细胞表达B7分子，刺激T淋巴细胞增生，使PBMC分泌IFN-γ增多，显著提高了白血病细胞的免疫原性。
Objective To investigate the effects of cytarabine（Ara-C） on expression of B7 molecules and immunogenicity of acute leukemia （AL） cells. Methods The expression of B7 molecules on fresh AL cells and on the Ara-C exposed leukemia cells was detected by FACS cytometer. B7 mRNA in Ara-C treated HL-60 cells were detected by reverse RT-PCR. The stimulation of proliferation of allogeneic PBMC by Ara-C treated HL-60 cells was detected by MTT method. Results B7-2 was weakly expressed in four and posifive in three, whereas B7-1 was posifive in only one of fourty AL patiens. Ara-C significantly enhanced B7 molecules expression on AL cells . Ara-C could induce higher expression of B7 mRNAs on HL-60 cells. Ara-C treated HL-60 cells could stimulate PBMC proliferation and promote their IFN-γ production. Conclusion Fresh AL cells express low level of B7 molecules. Ara-C enhances the B7 molecules expression on AL cells. The Ara-C treated leukemia cells can significantly stimulate the proliferation of allogeneic PBMC and induce their secretion of IFN-γ.
出处
《白血病．淋巴瘤》
CAS
2009年第11期650-653,共4页
Journal of Leukemia & Lymphoma
基金
国家自然科学基金资助（30701012）
天津市应用基础研究计划（07JCZDJ04900）
关键词
白血病
急性
阿糖胞苷
共刺激分子
免疫性
Leukemia, acute
Cytarabine
Molecule costimulatory
Immunogenicity
