摘要
目的:分析湖北地区宫颈癌组织HPV16E7和E5基因序列变异情况。方法:对87例宫颈鳞癌组织分别采用多重HPV16E7引物进行巢式PCR扩增和HPV16E5特异引物进行PCR扩增,并选择阳性扩增的基因片段DNA进行纯化、测序,检测基因变异。结果:测序成功的20例宫颈癌组织DNA中,HPV16E7基因的5个突变位点为:T846C、A647G、T732C、T789C、T795G,突变频率分别为85%(17/20)、70%(14/20)、15%(3/20),10%(2/20)、5%(1/20);HPV16E5基因的4个突变位点包括:A3979C、A4042G、G3868A、C3991T,突变频率分别为:80%(16/20)、90%(18/20)、5%(1/20)、5%(1/20)。结论:湖北地区宫颈癌的宫颈组织中HPV16E7热点突变为A647G和T846C,HPV16E5的热点突变为A3979C和A4042G,本研究为湖北地区宫颈癌流行病学的研究打下基础。
Objective：To identify the sequence variations in the HPV 16 E7 and E5 genes among patients with cervical cancer in Hubei province of China. Methods：Eighty-seven patients with cervical cancer were involved in this study. Using polymerase chain reaction （PCR） and PCR-directed sequencing methods,the variations of the HPV 16 E7 and E5 genes were investigated. Results：The rank orders of incidence of HPV16 E7 variants from cervical cancer in Hubei were as follows：T846C in 85% （17/20）,A647G in 70% （14/20）,T732C in 15% （3/20）,T789C in 10% （2/20）,and T795G in 5% （1/20）. There are four points of mutation in E5 gene,including A3979C,A4042G,G3868A,and C3991T with mutant frequencies of 80%（16/20）,90%（18/20）,5%（1/20）,and 5%（1/20）,respectively. Conclusion：The hot-spot mutations of HPV16 from cervical cancer in Hubei are A647G and T846C in E7 gene,and A3979C and A4042G in HPV16 E5 gene. The characterization of sequence variations within high-risk HPV 16 might be important in the search for epidemiological cervical cancer risk factors.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2009年第6期759-762,I0002,共5页
Medical Journal of Wuhan University
基金
湖北省卫生厅科研基金重点项目(编号:JX3A17)
关键词
宫颈癌
HPV16
E7和E5基因
基因多态性
Human Papillomavirusl6 （HPV16）
Cervix Cancer
E7 and E5 Genes~ Polymorphism
