摘要
目的:探讨雌二醇和孕激素水平与免疫功能变化在子痫前期患者发病中作用。方法:选取26例子痫前期患者,采用流式细胞术检测其外周血中T淋巴细胞及其亚群的数量;采用酶联免疫吸附法(enzyme-linked immunosorbant assay,ELISA)测定其血清中细胞因子干扰素-γ(interferon-γ,IFN-γ)和白介素-4(interleukin-4,IL-4)的浓度;用化学发光法检测其血清中雌二醇和孕激素的水平;同时选取同期正常妊娠妇女16例和12例非妊娠健康妇女检测上述各项指标作为对照。结果:子痫前期患者外周血中CD3+细胞和CD4+细胞的百分率以及CD4+/CD8+的比值均显著高于正常妊娠妇女(P<0.05);②子痫前期患者血清中IFN-γ浓度明显高于正常妊娠妇女和正常育龄妇女(P<0.05),而IL-4显著减少;③子痫前期患者血清中雌二醇与孕激素水平明显低于正常妊娠妇女。结论:子痫前期患者细胞免疫功能增强,这些免疫参数的改变与雌二醇与孕激素水平的降低有密切的关系,因此雌二醇与孕激素可能是调控妊娠妇女免疫功能改变的重要因素之一。
Objective： To explore the values of estradiol, progesterone levels and changes of immune function in pathogenesis of preeclampsia. Methods ： 26 patients with preeclampsia were selected, the amounts of T lymphocytes and subgroups in peripheral blood were detected by flow cytometry ; the levels of interferon - γ （ IFN - γ） and interlenkin - 4 （ IL - 4） in serum were measured by enzyme - linked immunosorbant assay （ELISA） ; the levels of estradiol and progesterone were detected by chemiluminescent microparticle immunoassay; 16 healthy pregnant women and 12 nongestational women were selected to detect above -mentioned parameters simultaneously. Results： ①The proportions of CD3 ^＋ T cells and CD4 ^＋ T ceils and the ration of CD4 ^＋/CD8 ^＋ in patients with preeclampsia were higher than those in healthy pregnant women （ P 〈 0. 05 ） , ②The level of IFN -γ in patients with preeclampsia was higher than those in healthy pregnant women and nongestational women （ P 〈 0. 05 ）, but the level of IL - 4 reversed. ③The levels of estradiol and progesterone in patients with preeclampsia were lower than those in healthy pregnant women. Conclusion： Immune function of patients with preeclampsia increase, the changes of immune parameters are related to the reduction Of estradiol and progesterone, estradiol and progesterone maybe crucial effect factors of immune function of patients with preeclampsia.
出处
《中国妇幼保健》
CAS
北大核心
2009年第33期4735-4737,共3页
Maternal and Child Health Care of China
基金
镇江市科技局社会发展项目(SH2007040)
